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Tissue-Specific Stem Cells
Version of Record online: 28 MAY 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 9, pages 2220–2228, September 2009
How to Cite
Burger, P. E., Gupta, R., Xiong, X., Ontiveros, C. S., Salm, S. N., Moscatelli, D. and Wilson, E. L. (2009), High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. STEM CELLS, 27: 2220–2228. doi: 10.1002/stem.135
Author contributions: P.B., E.L.W.: conception and design; P.B., R.G., X.X., C.O., S.S.: collection and assembly of data; P.B., E.L.W.: data analysis and interpretation; P.B., D.M., E.L.W.: manuscript writing; P.B., R.G., X.X., C.O., S.S., D.M., E.L.W.: final approval of manuscript.
First published online in STEM CELLS EXPRESS May 28, 2009.
- Issue online: 8 SEP 2009
- Version of Record online: 28 MAY 2009
- Accepted manuscript online: 28 MAY 2009 12:00AM EST
- Manuscript Accepted: 17 MAY 2009
- Manuscript Received: 6 FEB 2009
- University of Cape Town Staff Research Fund
- South African Medical Research Council
- National Institutes of Health. Grant Number: CA132641
- New York State Department of Health, Amgen Inc.
- Center for Stem Cell Biology at the New York University School of Medicine
- Adult stem cells;
- Fluorescence activated cell sorter;
- Progenitor cells;
We have shown previously that prostatic stem/progenitor cells can be purified from isolated prostate ducts, based on their high expression of the Sca-1 surface antigen. We now report that high levels of aldehyde dehydrogenase (ALDH) activity are present in a subset of prostate epithelial cells that coexpress a number of antigens found on stem/progenitor cells of other origins (CD9, Bcl-2, CD200, CD24, prominin, Oct 3/4, ABCG2, and nestin). Almost all of these cells expressing high levels of ALDH activity also express Sca-1 and a third of them express high levels of this antigen. The cells with high levels of ALDH activity have greater in vitro proliferative potential than cells with low ALDH activity. Importantly, in an in vivo prostate reconstitution assay, the cells expressing high levels of ALDH activity were much more effective in generating prostatic tissue than a population of cells with low enzymatic activity. Thus, a high level of ALDH activity can be considered a functional marker of prostate stem/progenitor cells and allows for simple, efficient isolation of cells with primitive features. The elucidation of the role of ALDH in prostate stem/progenitor cells may lead to the development of rational therapies for treating prostate cancer and benign prostatic hyperplasia. STEM CELLS 2009;27:2220–2228