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Tissue-Specific Stem Cells
Version of Record online: 28 MAY 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 9, pages 2220–2228, September 2009
How to Cite
Burger, P. E., Gupta, R., Xiong, X., Ontiveros, C. S., Salm, S. N., Moscatelli, D. and Wilson, E. L. (2009), High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. STEM CELLS, 27: 2220–2228. doi: 10.1002/stem.135
Author contributions: P.B., E.L.W.: conception and design; P.B., R.G., X.X., C.O., S.S.: collection and assembly of data; P.B., E.L.W.: data analysis and interpretation; P.B., D.M., E.L.W.: manuscript writing; P.B., R.G., X.X., C.O., S.S., D.M., E.L.W.: final approval of manuscript.
First published online in STEM CELLS EXPRESS May 28, 2009.
- Issue online: 8 SEP 2009
- Version of Record online: 28 MAY 2009
- Accepted manuscript online: 28 MAY 2009 12:00AM EST
- Manuscript Accepted: 17 MAY 2009
- Manuscript Received: 6 FEB 2009
- University of Cape Town Staff Research Fund
- South African Medical Research Council
- National Institutes of Health. Grant Number: CA132641
- New York State Department of Health, Amgen Inc.
- Center for Stem Cell Biology at the New York University School of Medicine
Additional supporting information available online.
|STEM_135_sm_suppinfofig1.tif||459K||Fig. S1. ALDH hi and ALDH lo cells are of similar size. Phase contrast images of ALDH hi cells (A) and ALDH lo cells (B) show that the cell populations having different ALDH activities have little or no difference in size. (Scale bars = 10 μm).|
|STEM_135_sm_suppinfofig2.tif||1036K||Fig. S2. The proximal region of prostatic ducts is enriched in ALDH hi cells. Cell digests from the proximal region of prostatic ducts (A, B) or the whole prostate (all regions of ducts) (C, D) were incubated with Aldefluor in the presence (A, C) or absence (B, D) of the inhibitor, DEAB. High levels of ALDH activity were expressed by 18.6% of proximal cells (B). Plots of proximal prostate cells are representative of 4 experiments. Cell digests from the whole prostate that contained 8.9% ALDH hi cells (D). Plots of whole prostate are representative of 7 experiments.|
|STEM_135_sm_suppinfofig3.tif||1820K||Fig. S3. FACS analysis of surface antigens expressed by ALDH hi and ALDH lo cells. Cells were incubated with Aldefluor substrate (this substrate is converted into a green fluorescent product by ALDH that can be measured by FACS in the FL1 channel) and subsequently incubated with biotinylated antibodies against EpCam, Sca-1, CD9, CD200, CD24 or prominin, followed by SA-APC. Controls, using appropriate biotinylated antibodies, were included for each antigen. The ALDH hi and ALDH lo populations were gated and analysed for antigen expression. Dot plots indicate the percentage of ALDH hi or ALDH lo cells that express the indicated antigens and include their respective isotype controls. Each plot is a representative example of more than one experiment.|
|STEM_135_sm_suppinfofig4.tif||1616K||Fig. S4. FACS analysis of intracellular antigens expressed by ALDH hi and ALDH lo cells. Cells were incubated with Aldefluor substrate and subsequently FACS sorted into ALDH hi and ALDH lo cells. The cells from each population were then permeabilized and incubated with polyclonal antibodies against CK5, ALDH1/2, ALDH3A1, Bcl-2, Oct 3/4, ABCG2 or nestin, followed by goat anti-rabbit APC or monoclonal antibodies against CK8, followed by rabbit anti-mouse RPE-Cy5. Appropriate isotype control antibodies were included for each antigen. Histograms indicate the percentage of ALDH hi or ALDH lo cells that express the indicated antigens. Each plot is a representative example of more than one experiment.|
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