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Tissue-Specific Stem Cells
Version of Record online: 22 MAY 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 6, pages 1170–1180, June 2013
How to Cite
Sun, D., Junger, W. G., Yuan, C., Zhang, W., Bao, Y., Qin, D., Wang, C., Tan, L., Qi, B., Zhu, D., Zhang, X. and Yu, T. (2013), Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors. STEM CELLS, 31: 1170–1180. doi: 10.1002/stem.1356
Author contributions: D.S.: performed experiments and wrote the manuscript; W.G.J.: helped with study design and finalizing manuscript; C.Y.: provided administrative support; W.Z.: provided study material; Y.B.: prepared figures; D.Q. and C.W.: prepared samples; L.T. and B.Q.: performed experiments and collected data; D.Z. and X.Z.: provided administrative support; T.Y.: Developed concept, designed the study, provided overall guidance, and finalized manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
first published online in STEM CELLS EXPRESS February 13, 2013.
- Issue online: 22 MAY 2013
- Version of Record online: 22 MAY 2013
- Accepted manuscript online: 13 FEB 2013 06:18AM EST
- Manuscript Accepted: 29 JAN 2013
- Manuscript Revised: 13 JAN 2013
- Manuscript Received: 16 OCT 2012
- National Natural Science Foundation of China. Grant Numbers: 81172183, 30500132, 31270202, 10832012, 11272134
Additional Supporting Information may be found in the online version of this article.
|sc-12-0895_sm_SupplFigure1.pdf||264K||Shockwaves cause osteogenic differentiation of hMSCs Suppl. Fig. 1. Silencing of P2X7 receptor expression. (A) hMSCs were treated with nonsense control siRNA or siRNA targeting P2X7 receptors for the indicated duration and P2X7 receptor mRNA levels were determined with real-time RT-PCR. Data represent means ± SE; *p<0.05; n≥3; two-tailed unpaired Student's t test, compared to controls. (B) After cells were treated with nonsense control siRNA or P2X7 receptor-targeting siRNA for 72 h, the expression of P2X7 receptors was assessed by immunofluorescence staining using P2X7 receptor antibodies followed by fluorescence–labeled secondary antibodies. They were examined under a magnification of 400.|
|sc-12-0895_sm_SupplTable1.pdf||157K||Supplementary Table 1.|
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