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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 22 MAY 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 6, pages 1213–1217, June 2013
How to Cite
Li, Y., Yu, W., Cooney, A. J., Schwartz, R. J. and Liu, Y. (2013), Brief Report: Oct4 and Canonical Wnt Signaling Regulate the Cardiac Lineage Factor Mesp1 Through a Tcf/Lef-Oct4 Composite Element. STEM CELLS, 31: 1213–1217. doi: 10.1002/stem.1362
Author contributions: Y.L.: collection and/or assembly of data and data analysis and interpretation; W.Y.: collection and/or assembly of data; A.J.C.: provision of study material or patients; R.J.S.: data analysis and interpretation; Y.L.: collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
first published online in STEM CELLS EXPRESS February 4, 2013.
- Issue published online: 22 MAY 2013
- Article first published online: 22 MAY 2013
- Accepted manuscript online: 18 FEB 2013 04:34AM EST
- Manuscript Accepted: 29 JAN 2013
- Manuscript Received: 12 DEC 2012
- Texas Heart Institute
- University of Houston
- American Heart Association
- Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
- Embryonic stem cells;
- Progenitor cells;
- Cardiac myocytes
Oct4 is the gatekeeper of stem cell pluripotency, but recent evidences also support Oct4 as a key regulator of germ layer formation and lineage commitment. How Oct4 contributes to lineage commitment is not well understood. We identified a Tcf/Lef-Oct4 composite site in the promoter of the cardiac mesoderm gene Mesp1, with a nucleotide sequence identical to the previously established Sox2-Oct4 composite site. This Tcf/Lef-Oct4 composite site mediated synergistic activation of the Mesp1 promoter by Oct4 and canonical Wnt signaling. Transcription ternary complexes were formed with Oct4 and Wnt terminal components, Lef1. Point mutations on the Tcf/Lef-Oct4 composite site impaired Oct4 and Lef1 binding and Mesp1-β-gal transgene reporter expression during mouse embryogenesis. In ZHBTc4 murine embryonic stem cells, the loss of Oct4 during differentiation impaired Mesp1 expression and the development of the cardiac program. This Tcf/Lef-Oct4 composite site appears to be a unique nodal point regulatory element that may drive pluripotency via Sox2-Oct4 and switch on lineage-related genes through Oct4's recruitment of Tcf/Lef factors. STEM Cells 2013;31:1213–1217