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Tissue-Specific Stem Cells
Article first published online: 22 MAY 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 6, pages 1193–1201, June 2013
How to Cite
Cai, Y., Zhang, Y., Shen, Q., Rubenstein, J. L. R. and Yang, Z. (2013), A Subpopulation of Individual Neural Progenitors in the Mammalian Dorsal Pallium Generates Both Projection Neurons and Interneurons In Vitro. STEM CELLS, 31: 1193–1201. doi: 10.1002/stem.1363
Abbreviations: IN, interneuron-like cells; PN, projection neuron-like cells.
Author contributions: Y.C.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; Y.Z.: conception and design, collection and assembly of data, and data analysis and interpretation; Q.S.: data analysis and interpretation; J.L.R.: data analysis and interpretation and manuscript writing; Z.Y.: conception and design, data analysis and interpretation, financial support, and manuscript writing. Y.C. and Y.Z. contributed equally to this work.
Disclosure of potential conflicts of interest is found at the end of this article.
first published online in STEM CELLS EXPRESS February 4, 2013.
- Issue published online: 22 MAY 2013
- Article first published online: 22 MAY 2013
- Accepted manuscript online: 18 FEB 2013 04:34AM EST
- Manuscript Accepted: 1 FEB 2013
- Manuscript Received: 12 AUG 2012
- National Basic Research Program of China. Grant Numbers: 2011CB504400, 2010CB945500
- National Natural Science Foundation of China. Grant Numbers: 30990261, 31121061, 91232723
- Shanghai Shu-Guang. Grant Number: 09SG05
- Neural stem/progenitor cells;
- Projection neurons;
There are two major classes of neurons in nervous systems: projection neurons and interneurons. During Drosophila nervous system development, a subpopulation of individual stem/progenitor cells gives rise to both motor neurons and interneurons. However, it remains unknown whether individual stem/progenitor cells in the mammalian brain also have the potential to give rise to both projection neurons and interneurons. Here we present evidence that single mouse neocortical progenitors generated both projection neurons and GABAergic interneurons based on studies using fluorescence-activated cell sorting (to obtain individual progenitors) and in vitro clonal analysis using time-lapse video microscopy and immunostaining. We determined that a subpopulation of individual dorsal pallial progenitors from E11.5 Dlx5/6-cre-IRES-EGFP and GAD67-GFP mice can generate both GFP-negative/Tbr1-positive (GFP−/Tbr1+)/Tuj1+ cells and GFP+/Sp8+/calretinin+/Tuj1+ cells. The GFP−/Tbr1+/Tuj1+ cells had morphological features of cultured projection neurons. Quantitative analysis of the reconstructed lineage trees derived from single progenitors showed that the projection neuron lineage appeared earlier than the interneuron lineage; however, more interneuron-like cells were produced than projection neuron-like cells. Thus, our results provide direct in vitro evidence that individual progenitors of the mammalian dorsal pallium can generate both projection neurons and interneurons. STEM Cells 2013;31:1139–1201