SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
sc-12-0633_sm_SupplFigure1.pdf1873KSupplementary Figure 1. BMMC Characterization. BMMC population was characterized for hematopoietic cells and MSC. A) Using CD45 and CD14 hematopoietic marker, we identified 65% of non hematopoietic cells after 2 passages in vitro. Gated cells were positive for CD73 (B) CD90 (C) and CD105 (D). Selected CD45-CD14 negative cells were plated in adipogenic (E) and osteogenic (F) media, were allowed to differentiate and compared to non treated MSC controls. Representative images of flow cytometry analysis and MSC differentiation. Scale bar = 50 μm
sc-12-0633_sm_SupplFigure2.pdf1876KSupplementary Figure 2. MSC do not transdifferentiate into TM cells in vitro. A) TM cells and MSC before and after 1 week co-culture with TM cells were assessed for the presence of laminin, fibronectin, aquaporin-1 and Pax6 by flow cytometry. Fluorescence intensity was measured on cell tracker positive MSC (dot plot). B) Compilation of mean fluorescence intensity for each antigen in TM cells, MSC alone and MSC co-cultured with TM. (Mean±SEM; n=3 from 3 distinct experiments). Significance: *p<0.05, ***p<0.001.
sc-12-0633_sm_SupplFigure3.pdf1570KSupplementary Figure 3. MSC phagocytosis by ocular microglia. Pictures are representative images of day 2 post laser damage tissue sections that were stained with microglia marker F 4/80 after A) injection of saline solution or B) injection with CFSE+ MSC. Scale bar = 50 μm
sc-12-0633_sm_SupplFigure4.tif1261KSupplementary Figure 4. MSC paracrine factors promote restoration of TM function. Aqueous humor clearance was evaluated by measuring peripheral blood radioactive tracer levels in counts per minutes (CPM) at different time points after injection of radiotracer in normal eyes (black line, n=3), laser treated eyes (blue line, n=3) and low oxygen CM treated eyes (red line, n=3). Results are expressed as mean±SEM of 2 independent experiments. Statistical significance was observed in low O2 CM exposed eyes versus laser only and normal controls: *p<0.05
sc-12-0633_sm_SupplFigure5.pdf1629KSupplementary Figure 5. Decreased IOP allows for better survival of retinal cells Histological sections of the retinal area from a rat eye stained by TUNEL assay. Pictures are representative images of retinas from a A) untreated control B) Laser + normoxic CM treated eye, day 12 C) laser + low oxygen treated eye, day 12. D) Compilation of total number of TUNEL+ cells present in the retina 12 days after laser damage and CM injection. Cell numbers (mean±SEM) were determined by fluorescence microscopy analysis of sequential ocular sections (n=3 per time point). *p<0.05. Scale bar = 100 μm
sc-12-0633_sm_SupplFigure6.pdf1656KSupplementary Figure 6. Nestin expression and cellular proliferation in the damaged and intact areas. Graphs represent compilation of nestin (A-B) and Ki67 (C-D) expressing cells based on their location: Intact area (A-C) and damaged area (B-D). Three animals per group from 2 separate experiments (N=6). Numbers indicate the percentage of positive cells found in the area composed of the ciliary body, the TM and a limited portion of the iris.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.