Native Adipose Stromal Cells Egress from Adipose Tissue In Vivo: Evidence During Lymph Node Activation§

Authors

  • Marta Gil-Ortega,

    1. CNRS, Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    2. EFS (Etablissement Français du Sang), STROMAlab BP 84225, Toulouse, France
    3. Inserm U1031 STROMAlab BP 84225, Toulouse, France
    4. Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    5. I2MC, Inserm 1048, Toulouse, France
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  • Lucile Garidou,

    1. I2MC, Inserm 1048, Toulouse, France
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  • Corinne Barreau,

    1. CNRS, Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    2. EFS (Etablissement Français du Sang), STROMAlab BP 84225, Toulouse, France
    3. Inserm U1031 STROMAlab BP 84225, Toulouse, France
    4. Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
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  • Marie Maumus,

    1. I2MC, Inserm 1048, Toulouse, France
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  • Ludovic Breasson,

    1. I2MC, Inserm 1048, Toulouse, France
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  • Geneviève Tavernier,

    1. I2MC, Inserm 1048, Toulouse, France
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  • Concha F. García-Prieto,

    1. Departamento de Ciencias Farmacéuticas y de la Alimentación, Facultad de Farmacia, Universidad CEU San Pablo, Madrid, Spain
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  • Anne Bouloumié,

    1. I2MC, Inserm 1048, Toulouse, France
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  • Louis Casteilla,

    1. CNRS, Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    2. EFS (Etablissement Français du Sang), STROMAlab BP 84225, Toulouse, France
    3. Inserm U1031 STROMAlab BP 84225, Toulouse, France
    4. Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
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  • Coralie Sengenès

    Corresponding author
    1. CNRS, Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    2. EFS (Etablissement Français du Sang), STROMAlab BP 84225, Toulouse, France
    3. Inserm U1031 STROMAlab BP 84225, Toulouse, France
    4. Université Toulouse III, UPS UMR5273 STROMAlab, BP 84225, Toulouse, France
    5. I2MC, Inserm 1048, Toulouse, France
    • StromaLab UMR UPS/CNRS/EFS 5273, Inserm U1031, Hôpital Rangueil, Bâtiment L1, BP 84225, 31432 Toulouse, France
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    • Telephone: +33-5-61-28-37-71; Fax: +33-5-62-17-09-05


  • Author contributions: M G-O., L.G., and C.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; C.B., M.M., L.B., G.T., and C.F.G-P.: collection and/or assembly of data and final approval of manuscript; A.B.: financial support, data analysis and interpretation, and final approval of manuscript; L.C.: conception and design, financial support, data analysis and interpretation, manuscript writing, and final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    first published online in STEM CELLS EXPRESS March 26, 2013.

Abstract

Adipose tissue (AT) has become accepted as a source of multipotent progenitor cells, the adipose stromal cells (ASCs). In this regard, considerable work has been performed to harvest and characterize this cell population as well as to investigate the mechanisms by which transplanted ASCs mediate tissue regeneration. In contrast the endogenous release of native ASCs by AT has been poorly investigated. In this work, we show that native ASCs egress from murine AT. Indeed, we demonstrated that the release of native ASCs from AT can be evidenced both using an ex vivo perfusion model that we set up and in vivo. Such a mobilization process is controlled by CXCR4 chemokine receptor. In addition, once mobilized from AT, circulating ASCs were found to navigate through lymph fluid and to home into lymph nodes (LN). Therefore, we demonstrated that, during the LN activation, the fat depot encapsulating the activated LN releases native ASCs, which in turn invade the activated LN. Moreover, the ASCs invading the LN were visualized in close physical interaction with podoplanin and ER-TR7 positive structures corresponding to the stromal network composing the LN. This dynamic was impaired with CXCR4 neutralizing antibody. Taken together, these data provide robust evidences that native ASCs can traffic in vivo and that AT might provide stromal cells to activated LNs. STEM Cells2013;31:1309–1320

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