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Tissue-Specific Stem Cells
Version of Record online: 5 JUL 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 7, pages 1371–1382, July 2013
How to Cite
Liu, O., Xu, J., Ding, G., Liu, D., Fan, Z., Zhang, C., Chen, W., Ding, Y., Tang, Z. and Wang, S. (2013), Periodontal Ligament Stem Cells Regulate B Lymphocyte Function via Programmed Cell Death Protein 1. STEM CELLS, 31: 1371–1382. doi: 10.1002/stem.1387
Author contributions: O.L., J.X., and S.W.: designed the study and wrote the manuscript; O.L., J.X., G.D., D.L., and S.W.: performed research and analyzed the data; Z.F.; C.Z., Y.D., and Z.T.: analyzed the data and technical support; W.C.: provided critical input on the immunological studies; S.W.: got funding and approved the manuscript. O.L. and J.X. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
first published online in STEM CELLS EXPRESS April 3, 2013.
- Issue online: 5 JUL 2013
- Version of Record online: 5 JUL 2013
- Accepted manuscript online: 3 APR 2013 05:25AM EST
- Manuscript Accepted: 7 MAR 2013
- Manuscript Revised: 19 FEB 2013
- Manuscript Received: 24 OCT 2012
- Beijing Municipal Committee for Science and Technology. Grant Number: Z121100005212004
- National Basic Research Program of China. Grant Number: 2010CB944801
- Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality. Grant Number: PHR20090510
- National Natural Science Foundation of China. Grant Number: NSFC NO.81070799
- Science Facility in Institutions of Higher Learning Under
- jurisdiction of Beijing Municipality. Grant Number: PXM 2009-014226-074691
- Intramural Research program of the National Institute of Dental
- Craniofacial Research, National Institutes of Health
Additional Supporting Information may be found in the online version of this article.
|sc-12-0998_sm_SupplFigure1.tif||2027K||Supplementary Figure 1. Identification of stem cell properties of hPDLSCs. (A): hPDLSCs were positive for STRO-1, CD146, CD90, SSEA-4, OCT-4 and CD73. (B): To detect osteogenic differentiation, calcification of the extracellular matrix was checked at the 5 day by alkaline phosphatase (ALP) staining and at the 14 day by via von Kossa staining. Oil red O staining was used to identify lipid-laden fat cells. (C): TO detect the purify of B cells, positively selected cells contained on average 93.28% B cells, as assessed by flow cytometric analysis with a CD19 PE monoclonal antibody.|
|sc-12-0998_sm_SupplFigure2.tif||2508K||Supplementary Figure 2. B cells in periodontal tissues 12 weeks after PDLSCs treatment. 12 weeks post-transplantation, more B cells were found in the control group (A) and HA/βTCP group (B), and a fewer B cells were observed in autologous (C) and allogeneic (D) group, and CD146 positive cells showed a contrary trend. B cells were stained using anti-CD19 antibody, bar = 50μm.|
|sc-12-0998_sm_SupplFigure3.tif||1481K||Supplementary Figure 3. Soluble factors determination in supernatants. (A-H) The results showed that there were no significant differences on the concentrations of PGE2, IL-2, IL-4, IL-10, IL-12p70, TNFα, IFNγ and TGFβ in the supernatants at the first day, the second day and the third day. (I) The proliferation index of stimulated B cells from miniature pigs was significantly higher than B cells co-cultured with both autologous and allogeneic PDLSCs (*P < 0.01 ). There was no difference between autologous and allogeneic group.|
|sc-12-0998_sm_SupplTable1.tif||961K||Supplementary Table 1. Routine blood and biochemical tests in the swine experiments. (A, B): There were no significant differences on routine blood and biochemical tests in whole blood in allogeneic groups based on the analysis of the samples after transplantation at different time points.|
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