Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells

Authors

  • Adam D. Gracz,

    1. Department of Medicine Division of Gastroenterology and Hepatology, The University of North Carolina at Chapel Hill, North Carolina, USA
    2. Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, North Carolina, USA
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  • Megan K. Fuller,

    1. Department of Surgery, The University of North Carolina at Chapel Hill, North Carolina, USA
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  • Fengchao Wang,

    1. Stowers Institute for Medical Research, Kansas City, USA
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  • Linheng Li,

    1. Stowers Institute for Medical Research, Kansas City, USA
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  • Matthias Stelzner,

    1. Department of Surgery, VA Greater Los Angeles Healthcare System, Los Angeles, California, USA
    2. Department of Surgery University of California, Los Angeles, California, USA
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  • James C.Y. Dunn,

    1. Department of Surgery University of California, Los Angeles, California, USA
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  • Martin G. Martin,

    1. Department of Pediatrics Division of Gastroenterology, University of California, Los Angeles, California, USA
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  • Scott T. Magness

    Corresponding author
    1. Department of Medicine Division of Gastroenterology and Hepatology, The University of North Carolina at Chapel Hill, North Carolina, USA
    2. Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, North Carolina, USA
    3. Biomedical Engineering, The University of North Carolina at Chapel Hill, Chapel Hill, USA
    • Ph.D., University of North Carolina at Chapel Hill, 111 Mason Farm Rd., CB# 7032, MBRB Rm. 4337, Chapel Hill, North Carolina 27599, USA. E-mail: magness@med.unc.edu Telephone: 919-966-6816; Fax: 919-843-6899

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  • Author contributions: A.D.G. and S.T.M.: conceived and designed the study, wrote the manuscript, and carried out culture and gene expression experiments; M.K.F.: developed and optimized protocols for epithelial isolation and FACS; F.W. and L.L.: provided GSK-inhibitor conditions; M.S., J.C.Y.D., and M.G.M.: provided critical review of the manuscript. A.D.G. and M.K.F. contributed equally to this article.

Abstract

Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive “active” stem cells as well as HOPX positive “facultative” stem cells. Fluorescence-activated cell sorting enables differential enrichment of LGR5 (CD24−/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease. STEM Cells 2013;31:2024-2030

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