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Tissue-Specific Stem Cells
Human Placenta-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration in CCl4-Injured Rat Liver Model via Increased Autophagic Mechanism†
Article first published online: 23 AUG 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 8, pages 1584–1596, August 2013
How to Cite
Jung, J., Choi, J. H., Lee, Y., Park, J.-W., Oh, I.-H., Hwang, S.-G., Kim, K.-S. and Kim, G. J. (2013), Human Placenta-Derived Mesenchymal Stem Cells Promote Hepatic Regeneration in CCl4-Injured Rat Liver Model via Increased Autophagic Mechanism. STEM CELLS, 31: 1584–1596. doi: 10.1002/stem.1396
Author contributions: J.J.: collection and analysis of data, data interpretation, and manuscript drafting; J.H.C.: data interpretation and analysis of revision data; Y.L.: data interpretation and analysis of data; J.W.P. and I.H.O.: conception and critical discussion; S.G.H.: financial support and critical discussion; K.S.K.: data interpretation, critical discussion, and manuscript drafting; G.J.K.: conception and design, manuscript drafting, financial support, and final approval of manuscript.
- Issue published online: 23 AUG 2013
- Article first published online: 23 AUG 2013
- Accepted manuscript online: 17 APR 2013 06:21AM EST
- Manuscript Accepted: 19 MAR 2013
- Manuscript Revised: 11 MAR 2013
- Manuscript Received: 27 SEP 2012
- Korean Government (MEST). Grant Number: KRF-2011-0019610
Additional Supporting Information may be found in the online version of this article.
|STEM_1396_sm_SuppFig1.pdf||53K||Supplement Fig. 1. Alteration of necrosis and apoptosis in CCl4-treated rat hepatic epithelial cells (WB-F344) co-cultured with CP-MSCs. (A) To assess necrotic cells, LDH analysis was performed on WB-F344 cells with or without CCl4 treatment and co-cultured with CP-MSCs or WI-38 cells; a coculture- free condition was used as a sham control under normoxia and hypoxia. All reactions were performed in triplicate. Data are expressed as the mean ± SD of triplicate experiments. Significant differences were observed between normoxia and hypoxia (1% oxygen) (*, p < .05) and between coculturing with CP-MSCs and WI-38 (#, p < .05), compared to the co-culture-free system (§, p < .05). (B) To analyze apoptotic cells, caspase 3/7 ELISA analysis was performed on WB-F344 cells using the same conditions as described (A). All reactions were performed in triplicate. Data are expressed as the mean ± SD of triplicate experiments. Significant differences were depicted between normoxia and hypoxia (1% oxygen) (*, p < .05) and between co-culturing with CP-MSCs and WI-38 (#, p < .05), as compared to the co-culture-free system (§, p < .05). CP-MSCs, CP; WI-38, WI.|
|STEM_1396_sm_SuppTab1.pdf||132K||Supporting Information Table 1.|
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