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STEM_1396_sm_SuppFig1.pdf53KSupplement Fig. 1. Alteration of necrosis and apoptosis in CCl4-treated rat hepatic epithelial cells (WB-F344) co-cultured with CP-MSCs. (A) To assess necrotic cells, LDH analysis was performed on WB-F344 cells with or without CCl4 treatment and co-cultured with CP-MSCs or WI-38 cells; a coculture- free condition was used as a sham control under normoxia and hypoxia. All reactions were performed in triplicate. Data are expressed as the mean ± SD of triplicate experiments. Significant differences were observed between normoxia and hypoxia (1% oxygen) (*, p < .05) and between coculturing with CP-MSCs and WI-38 (#, p < .05), compared to the co-culture-free system (§, p < .05). (B) To analyze apoptotic cells, caspase 3/7 ELISA analysis was performed on WB-F344 cells using the same conditions as described (A). All reactions were performed in triplicate. Data are expressed as the mean ± SD of triplicate experiments. Significant differences were depicted between normoxia and hypoxia (1% oxygen) (*, p < .05) and between co-culturing with CP-MSCs and WI-38 (#, p < .05), as compared to the co-culture-free system (§, p < .05). CP-MSCs, CP; WI-38, WI.
STEM_1396_sm_SuppTab1.pdf132KSupporting Information Table 1.

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