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Tissue-Specific Stem Cells
Version of Record online: 5 JUL 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 7, pages 1422–1433, July 2013
How to Cite
Tornillo, G., Elia, A. R., Castellano, I., Spadaro, M., Bernabei, P., Bisaro, B., Camacho-Leal, M. d. P., Pincini, A., Provero, P., Sapino, A., Turco, E., Defilippi, P. and Cabodi, S. (2013), p130Cas alters the differentiation potential of mammary luminal progenitors by deregulating c-Kit activity. STEM CELLS, 31: 1422–1433. doi: 10.1002/stem.1403
Author contributions: G.T.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; A.R.E., M.S., P.B., B.B., A.P., and E.T.: collection and/or assembly of data; I.C. and A.S.: provision of study material or patients and data analysis and interpretation; P.P., M.d.P.C.-L., and P.D.: data analysis and interpretation; S.C.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
first published online in STEM CELLS EXPRESS April 17, 2013.
- Issue online: 5 JUL 2013
- Version of Record online: 5 JUL 2013
- Accepted manuscript online: 17 APR 2013 06:23AM EST
- Manuscript Accepted: 14 MAR 2013
- Manuscript Revised: 5 MAR 2013
- Manuscript Received: 7 NOV 2012
- AIRC. Grant Numbers: IG2008, IG2011
Additional Supporting Information may be found in the online version of this article.
|sc-12-1049_sm_SupplFigure1.tif||736K||Supplemental Figure 1. Expression of keratin 18, alpha-SMA and ERalpha in sorted cells. (A) Histograms show keratin 18 (left panel) and alpha-SMA (right panel) mRNA relative expression in sorted luminal and basal cells, as assessed by qRT-PCR analysis. (B) Histograms show ERalpha mRNA relative expression in unsorted and sorted luminal CD24+/High Sca-1- and CD24+/High Sca-1+ cells, as assessed by qRT-PCR analysis.|
|sc-12-1049_sm_SupplFigure2.tif||200K||Supplemental Figure 2. Primers for detection of MMTV-p130Cas transgene transcript. (A) RT-PCR showing p130Cas transgene (myc-p130Cas) expression. Total cDNA prepared from Wt and Tg mammary cells was used as template. Beta-actin expression is also included.|
|sc-12-1049_sm_SupplFigure3.tif||229K||Supplemental Figure 3. p130Cas overexpression alters the differentiation potential of ER- luminal progenitor cells. (A) Phase contrast images of colonies arising from 3D culture of luminal ER- (CD24+/High Sca-1-) cells purified from Wt (left panel) or Tg (middle panel) mammary glands. Images of colonies derived from basal (CD24+/Low Sca-1-) cells isolated from Wt glands are shown in the right panels. Scale bar, 100 micron. Images are representative of three independent experiments. (B) Representative images of immunofluorescence staining with antibodies to keratin 5 (green, K5) and keratin 18 (red, K18) of colonies arising from sorted Wt CD24+/High Sca-1- (top panels), Tg CD24+/High Sca-1- (middle panels) and Wt (CD24+/Low Sca-1-) basal cells (bottom panels). Nuclei were stained with DAPI (blue). Scale bar, 50 micron.|
|sc-12-1049_sm_SupplFigure4.tif||2202K||Supplemental Figure 4. p130Cas is downregulated during differentiation of KIM-2 cells. (A) KIM-2 cells were treated with prolactin and dexamethasone for the number of days indicated and probed for p130Cas and beta-casein mRNA expression (left and right panel, respectively) by qRT-PCR analysis. (B) KIM- 2 cells, treated as above, were analyzed for p130Cas protein expression by immunoblotting. Actin was used as loading control. Bars represent the means ± SEM of three independent experiments.|
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