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Additional Supporting Information may be found in the online version of this article.

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sc-12-1049_sm_SupplFigure1.tif736KSupplemental Figure 1. Expression of keratin 18, alpha-SMA and ERalpha in sorted cells. (A) Histograms show keratin 18 (left panel) and alpha-SMA (right panel) mRNA relative expression in sorted luminal and basal cells, as assessed by qRT-PCR analysis. (B) Histograms show ERalpha mRNA relative expression in unsorted and sorted luminal CD24+/High Sca-1- and CD24+/High Sca-1+ cells, as assessed by qRT-PCR analysis.
sc-12-1049_sm_SupplFigure2.tif200KSupplemental Figure 2. Primers for detection of MMTV-p130Cas transgene transcript. (A) RT-PCR showing p130Cas transgene (myc-p130Cas) expression. Total cDNA prepared from Wt and Tg mammary cells was used as template. Beta-actin expression is also included.
sc-12-1049_sm_SupplFigure3.tif229KSupplemental Figure 3. p130Cas overexpression alters the differentiation potential of ER- luminal progenitor cells. (A) Phase contrast images of colonies arising from 3D culture of luminal ER- (CD24+/High Sca-1-) cells purified from Wt (left panel) or Tg (middle panel) mammary glands. Images of colonies derived from basal (CD24+/Low Sca-1-) cells isolated from Wt glands are shown in the right panels. Scale bar, 100 micron. Images are representative of three independent experiments. (B) Representative images of immunofluorescence staining with antibodies to keratin 5 (green, K5) and keratin 18 (red, K18) of colonies arising from sorted Wt CD24+/High Sca-1- (top panels), Tg CD24+/High Sca-1- (middle panels) and Wt (CD24+/Low Sca-1-) basal cells (bottom panels). Nuclei were stained with DAPI (blue). Scale bar, 50 micron.
sc-12-1049_sm_SupplFigure4.tif2202KSupplemental Figure 4. p130Cas is downregulated during differentiation of KIM-2 cells. (A) KIM-2 cells were treated with prolactin and dexamethasone for the number of days indicated and probed for p130Cas and beta-casein mRNA expression (left and right panel, respectively) by qRT-PCR analysis. (B) KIM- 2 cells, treated as above, were analyzed for p130Cas protein expression by immunoblotting. Actin was used as loading control. Bars represent the means ± SEM of three independent experiments.
sc-12-1049_sm_SupplData.pdf26KSupplementary Data

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