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Cancer Stem Cells
CXC Chemokine Receptor 4 is Essential for Maintenance of Renal cell Carcinoma-Initiating Cells and Predicts Metastasis†
Version of Record online: 23 AUG 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 8, pages 1467–1476, August 2013
How to Cite
Gassenmaier, M., Chen, D., Buchner, A., Henkel, L., Schiemann, M., Mack, B., Schendel, D. J., Zimmermann, W. and Pohla, H. (2013), CXC Chemokine Receptor 4 is Essential for Maintenance of Renal cell Carcinoma-Initiating Cells and Predicts Metastasis. STEM CELLS, 31: 1467–1476. doi: 10.1002/stem.1407
Author contributions: M.G.: collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; D.C: collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; A.B.: data analysis and interpretation and final approval of manuscript; L.H., M.S., and B.M.: collection and/or assembly of data and final approval of manuscript; D.J.S.: provision of study material and final approval of manuscript; W.Z.: conception and design, data analysis and interpretation, financial support, manuscript writing, and final approval of manuscript, H.P.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript. W.Z. and H.P. are the co-last authors.
- Issue online: 23 AUG 2013
- Version of Record online: 23 AUG 2013
- Accepted manuscript online: 30 APR 2013 03:50AM EST
- Manuscript Accepted: 28 MAR 2013
- Manuscript Received: 28 NOV 2012
Additional Supporting Information may be found in the online version of this article.
|STEM_1407_sm_SuppFigure1.tif||1316K||Supplemental Figure S1: Expression of putative stem cell markers on RCC-26 and RCC-53 cells. Cells were stained with fluorochrome-coupled antibodies (solid lines) and analyzed by flow cytometry. Unstained cells served as negative control (stippled lines). The fraction in % of positive cells exceeding the maximal autofluorescence of unlabeled cells is indicated..|
|STEM_1407_sm_SuppFigure2.tif||669K||Supplemental Figure S2: Inhibition of RCC-26 and RCC-53 by pazopanib, sunitinib and sorafenib. Cells were cultured in the presence of pazopanib, sunitinib and sorafenib at the indicated concentrations for 72 h. Cell viability was determined in comparison to cultures treated with the solvent alone. The IC50 values are indicated by dotted lines. The experiments were performed thrice in triplicates. Results are shown as means and SEM.|
|STEM_1407_sm_SuppTable1.pdf||54K||Supplemental Table S1: List of oligonucleotide primers and PCR products.|
|STEM_1407_sm_SuppTable2.pdf||11K||Supplemental Table S2: Correlation of CXCR4 expression in primary RCC and clinicopathologic variables.|
|STEM_1407_sm_SuppTable3.pdf||47K||Supplemental Table S3: Multivariate analyses of overall survival in patients with RCC.|
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