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Additional Supporting Information may be found in the online version of this article.

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STEM_1417_sm_SuppFigure1.pdf8KFig. S1. ALKi derived iPS cells behave as normal stem cells. qRT-PCR determination of mRNA expression levels of ground state markers Rex1 and Fgf4, and differentiation markers Fgf5 and Brachyury (normalized to actin mRNA) in iPS cells derived by Dox induced OSKM expression in the presence of ALKi. Ground state markers are expressed (top two panels, black bars), but not the differentiation markers (bottom two panels, black bars). Removing LIF and ALKi induces differentiation as demonstrated by the downregulation of Rex1 and Fgf4 (top two panels, gray bars), and the upregulation of Fgf5 and Brachyury (bottom two panels, gray bars). Error bars display SEM, n=3.
STEM_1417_sm_SuppFigure2.pdf8KFig. S2. Polycomb knockdown derived iPS cells express pluripotency markers. qRT-PCR determination of mRNA expression levels of Oct4, Nanog and Sox2 (normalized to actin mRNA) in iPS cells derived by Dox induced OSKM expression combined with shRNA knockdown of Jmjd3 or Suz12. Expression the indicated pluripotency markers in Jmjd3 knockdown iPS cells are comparable to control (no target), whereas Suz12 knockdown iPS cells show an increase. Error bars display SEM, n=3.

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