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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Polycomb Determines Responses to Smad2/3 Signaling in Embryonic Stem Cell Differentiation and in Reprogramming†
Article first published online: 23 AUG 2013
Copyright © 2013 AlphaMed Press
Volume 31, Issue 8, pages 1488–1497, August 2013
How to Cite
Dahle, Ø. and Kuehn, M. R. (2013), Polycomb Determines Responses to Smad2/3 Signaling in Embryonic Stem Cell Differentiation and in Reprogramming. STEM CELLS, 31: 1488–1497. doi: 10.1002/stem.1417
Author contributions: Ø.D.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; M.R.K.: conception and design, data analysis and interpretation, financial support, and manuscript writing.
- Issue published online: 23 AUG 2013
- Article first published online: 23 AUG 2013
- Accepted manuscript online: 10 MAY 2013 08:28AM EST
- Manuscript Accepted: 14 MAR 2013
- Manuscript Received: 14 NOV 2012
- Intramural Research Program of the National Cancer Institute
- National Institutes of Health
- Department of Health and Human Services
Additional Supporting Information may be found in the online version of this article.
|STEM_1417_sm_SuppFigure1.pdf||8K||Fig. S1. ALKi derived iPS cells behave as normal stem cells. qRT-PCR determination of mRNA expression levels of ground state markers Rex1 and Fgf4, and differentiation markers Fgf5 and Brachyury (normalized to actin mRNA) in iPS cells derived by Dox induced OSKM expression in the presence of ALKi. Ground state markers are expressed (top two panels, black bars), but not the differentiation markers (bottom two panels, black bars). Removing LIF and ALKi induces differentiation as demonstrated by the downregulation of Rex1 and Fgf4 (top two panels, gray bars), and the upregulation of Fgf5 and Brachyury (bottom two panels, gray bars). Error bars display SEM, n=3.|
|STEM_1417_sm_SuppFigure2.pdf||8K||Fig. S2. Polycomb knockdown derived iPS cells express pluripotency markers. qRT-PCR determination of mRNA expression levels of Oct4, Nanog and Sox2 (normalized to actin mRNA) in iPS cells derived by Dox induced OSKM expression combined with shRNA knockdown of Jmjd3 or Suz12. Expression the indicated pluripotency markers in Jmjd3 knockdown iPS cells are comparable to control (no target), whereas Suz12 knockdown iPS cells show an increase. Error bars display SEM, n=3.|
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