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Additional Supporting Information may be found in the online version of this article.

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STEM_1420_sm_SuppFigure1.pdf155KSupplementary Figure 1. Phosphorylation of ERM proteins in GFAP-positive glial tube in the adult rostral migratory stream. (A-C) Immunofluorescence labeling of pERM (A, green) and a glial tube marker, GFAP (B, red) in the adult mouse RMS. Note that two signals were spatially separated in merged images (C). (D-F) large magnification images of pERM (D), GFAP (E), and z-stacked orthogonal image (F). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar=5 μm.
STEM_1420_sm_SuppFigure2.pdf85KSupplementary Figure 2. Phosphorylation of ERM proteins in chain migration of SVZ explants cultures. (A-C) Immunofluorescence labeling of pERM (A, green) and the neuroblast marker DCX (B, red) in neuroblasts from explants cultures. Merged signals shown in C. Scale bar=10 μm.
STEM_1420_sm_SuppFigure3.pdf81KSupplementary Figure 3. Phosphorylation of radixin in adult RMS. Adult RMS lysates from normal (A) or injured (B) mouse brain were precipitated with anti-ezrin or radixin antibody, and immunoblot was performed using anti-pERM antibody. pERM signals were predominantly seen in the samples immunoprecipitated with anti-radixin antobody.
STEM_1420_sm_SuppFigure4.pdf498KSupplementary Figure 4. Expression of SDF1 in activated microglia and CXCR4 in migrating neuroblasts after cryogenic traumatic brain injury (TBI). Schematic diagrams of the normal and injured brains, indicating the damaged regions and RMS, where microglia are activated, and neuroblasts expresses CXCR4. (A-F) Immunohistochemistry with SDF1 (red) and Iba1 (green), a microglia maker in the control (A-C) and injured mouse brain (D-F). (G-L) Immunohistochemistry with a SDF1 receptor, CXCR4 (red) and a neuroblast maker, DCX (green) in the RMS of control (G-I) and injured (J-L) mouse brain. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar=10 μm. Abbreviations are SVZ, subventricular zone; RMS, rostral migratory stream; OB, olfactory bulb.
STEM_1420_sm_SuppFigure5.pdf159KSupplementary Figure 5. Effects of DN-ERM on the survival and proliferation of neural stem/progenitor cells. Immunocytochemistry with BrdU (red) and GFP (A, C) or DN-ERM (B, D) infected neural stem cells. Three hours before fixation of NSCs, BrdU (20 μg/ml) was treated in both groups. Nuclei were counterstained with Hoechst 33342 (blue). Insets show magnified images of BrdU and GFP double positive cells (A-D). Quantification of the number of BrdU+/GFP+ cells demonstrate that DN-ERM did not change proliferation rate of stem cells (E). Immunohistochemistry with cleaved caspase3 (red) in GFP (F, H) or DNERM (G, I) injected mice. Insets show magnified images of cleaved caspase3 positive cells (F-I)
STEM_1420_sm_SuppFigure6.pdf135KSupplementary Figure 6. Altered morphology of RMS neuroblasts by DN-ERM in vivo. Retrovirus was injected into the SVZ of adult mice. Representative images of neuroblasts infected with EGFP (A, C) or DN-ERM (B, D) in the RMS. C and D include nuclear counterstaining by Hoechst33342 (blue). Scale bar= 10 μm.
STEM_1420_sm_SuppFigure7.pdf81KSupplementary Figure 7. Effect of DN-ERM on morphology of neuronal stem cells. Dissociated SVZ neural stem cells were infected with EGFP (A, C) or DN-ERM (B, D) viruses and seeded onto laminin-coated dishes. (A-D) Immunohistochemistry with nestin (red) and GFP. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar=50 μm.
STEM_1420_sm_SuppInfo.pdf68KSupporting Information

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