Expression of Podocalyxin Separates the Hematopoietic and Vascular Potentials of Mouse Embryonic Stem Cell-Derived Mesoderm

Authors

  • Hailan Zhang,

    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
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  • Johnathan L. Nieves,

    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
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  • Stuart T. Fraser,

    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
    3. The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA
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  • Joan Isern,

    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
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  • Panagiotis Douvaras,

    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
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  • Dmitri Papatsenko,

    1. The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA
    2. Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
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  • Sunita L. D'Souza,

    1. The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA
    2. Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
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  • Ihor R. Lemischka,

    1. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
    2. The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA
    3. Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
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  • Michael A. Dyer,

    1. Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York, USA
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  • Margaret H. Baron

    Corresponding author
    1. Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA
    2. The Tisch Cancer Institute, Mount Sinai School of Medicine, New York, New York, USA
    3. The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York, USA
    4. Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA
    5. Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA
    • Correspondence: Margaret H. Baron, M.D. Ph.D., Mount Sinai School of Medicine, Box 1079, 1468 Madison Avenue, Annenberg 24-04E, New York, New York 10029-6574, USA. Telephone: +1-212-241-0825; Fax: +1-212-849-2442; email: margaret.baron@mssm.edu

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Abstract

In the mouse embryo and differentiating embryonic stem cells, the hematopoietic, endothelial, and cardiomyocyte lineages are derived from Flk1+ mesodermal progenitors. Here, we report that surface expression of Podocalyxin (Podxl), a member of the CD34 family of sialomucins, can be used to subdivide the Flk1+ cells in differentiating embryoid bodies at day 4.75 into populations that develop into distinct mesodermal lineages. Definitive hematopoietic potential was restricted to the Flk1+Podxl+ population, while the Flk1-negative Podxl+ population displayed only primitive erythroid potential. The Flk1+Podxl-negative population contained endothelial cells and cardiomyocyte potential. Podxl expression distinguishes Flk1+ mesoderm populations in mouse embryos at days 7.5, 8.5, and 9.5 and is a marker of progenitor stage primitive erythroblasts. These findings identify Podxl as a useful tool for separating distinct mesodermal lineages. Stem Cells 2014;32:191–203

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