Embryonic Stem Cells/Induced Pluripotent Stem Cells

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Birth of Parthenote Mice Directly from Parthenogenetic Embryonic Stem Cells†‡

  1. Zhisheng Chen1,2,
  2. Zhong Liu1,
  3. Junjiu Huang1,3,
  4. Tomokazu Amano4,
  5. Chao Li1,
  6. Shanbo Cao1,
  7. Chao Wu1,
  8. Bodu Liu1,
  9. Lingjun Zhou1,
  10. Mark G. Carter4,
  11. David L. Keefe3,
  12. Xiangzhong Yang4,
  13. Lin Liu3,5,§,*

Article first published online: 19 JUN 2009

DOI: 10.1002/stem.158

Issue

STEM CELLS

STEM CELLS

Volume 27, Issue 9, pages 2136–2145, September 2009

Additional Information

How to Cite

Chen, Z., Liu, Z., Huang, J., Amano, T., Li, C., Cao, S., Wu, C., Liu, B., Zhou, L., Carter, M. G., Keefe, D. L., Yang, X. and Liu, L. (2009), Birth of Parthenote Mice Directly from Parthenogenetic Embryonic Stem Cells. STEM CELLS, 27: 2136–2145. doi: 10.1002/stem.158

Author Information

  1. 1

    School of Life Science, Sun Yet-Sen University, Guangzhou, China

  2. 2

    College of Life Science, Foshan University, Foshan, China

  3. 3

    Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, Florida

  4. 4

    Center for Regenerative Biology and Department of Animal Science, University of Connecticut, Storrs, Connecticut

  5. 5

    College of Life Sciences, Key Laboratory of Bioactive Materials of Ministry of Education, Nankai University, Tianjin, China

  1. §

    Telephone: 86-22-23500752

*College of Life Sciences, Nankai University, Tianjin 300071, China

  1. †

    Author contributions: L.L.: conception and design, financial support, final approval of manuscript; Z.C.: collection and assembly of data, data analysis and interpretation, manuscript writing; J.H.: collection and assembly of data, data analysis and interpretation; Z.L., C.L., B.L., C.W., S.C., T.A.: collection and assembly of data; L.Z.: provision of study material; M.G.C.: collection and assembly of data, data analysis and interpretation; D.L.K., X.Y., data analysis and interpretation, financial support, final approval of manuscript.

  2. ‡

    First published online in STEM CELLS EXPRESS June 19, 2009.

Publication History

  1. Issue published online: 8 SEP 2009
  2. Article first published online: 19 JUN 2009
  3. Accepted manuscript online: 19 JUN 2009 12:00AM EST
  4. Manuscript Accepted: 9 JUN 2009
  5. Manuscript Received: 22 MAR 2009

Funded by

  • National Natural Science Foundation, Science and Technology Division of Guangdong Province, and China Ministry of Science and Technology. Grant Number: 2009CB941000
  • USDA-ARS. Grant Numbers: AG 58-1265-2-018, 58-1265-2-020

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Keywords:

  • Parthenogenesis;
  • ESC line;
  • Tetraploid complementation;
  • Mice

Abstract

Mammalian parthenogenetic embryos are not viable and die because of defects in placental development and genomic imprinting. Parthenogenetic ESCs (pESCs) derived from parthenogenetic embryos might advance regenerative medicine by avoiding immuno-rejection. However, previous reports suggest that pESCs may fail to differentiate and contribute to some organs in chimeras, including muscle and pancreas, and it remains unclear whether pESCs themselves can form all tissue types in the body. We found that derivation of pESCs is more efficient than of ESCs derived from fertilized embryos, in association with reduced mitogen-activated protein kinase signaling in parthenogenetic embryos and their inner cell mass outgrowth. Furthermore, in vitro culture modifies the expression of imprinted genes in pESCs, and these cells, being functionally indistinguishable from fertilized embryo-derived ESCs, can contribute to all organs in chimeras. Even more surprisingly, our study shows that live parthenote pups were produced from pESCs through tetraploid embryo complementation, which contributes to placenta development. This is the first demonstration that pESCs are capable of full-term development and can differentiate into all cell types and functional organs in the body. STEM CELLS 2009;27:2136–2145

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