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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
stem1605-sup-0001-suppfig1.tif10411KSupplemental Figure S1. Definition and validation of the GASC-based score for the prognostic stratification of LGG patients. The picture recapitulates the workflow followed, the casistics used and the statistical tests adopted.
stem1605-sup-0002-suppfig2.tif2111KSupplemental Figure S2. Primary culture of glioma-derived cells. (A-D) Colony forming efficiency. (A-C) Phase contrast images of colonies formed few days after seeding glioma derived cells in the medium selective for the growth of multipotent adult stem cells. In (A) the inset at the bottom-right corner shows at higher magnification the region within the white rectangle. (D) Quantitative analysis of the colony-forming efficiency of cells derived from low-grade (n = 14) and high-grade (n = 18) glioma samples. (E-J) Undifferentiated phenotype of proliferating cells. Expression, by cells in primary culture, of vimentin (green fluorescence, E), nestin (red fluorescence, F), Oct-4 (yellow-fluorescence, G), Nanog (magenta fluorescence, H) and GFAP (cyan fluorescence, I). (J) Histograms show the fraction of LGG- (n = 14) and HGG- (n = 18) derived cells expressing the described proteins. In (D) and (J) data are presented as mean±SD; *, p [lt] .05 versus low-grade glioma.
stem1605-sup-0003-suppfig3.tif1273KSupplemental Figure S3. (A-B) Mesodermic differentiation. GASC cultured in osteogenic medium present calcium deposits as assessed by von Kossa staining (brown deposits, A). In myogenic medium, GASC expressed smooth muscle actin filaments (SMA, red fluorescence, B). (C-D) Endodermic differentiation. A small fraction of GASC cultured in hepatocyte-inducing conditions became positive for cytokeratins 8-18-19 (green fluorescence, C) and were able to actively up-take DiI-labeled acetylated LDL (red fluorescence, D). (E) Capacity of GASC to differentiate into the different cell lineages. Results are expressed as mean ± S.D.
stem1605-sup-0004-suppfig4.tif279KSupplemental Figure S4. GASC medium modify A172 and U87 population doubling time (A), growth in soft agar (B). Data are presented as mean ± S.D.
stem1605-sup-0005-suppfig5.tif1258KSupplemental Figure S5. Representative dot-plots showing the expression of CD133, CD271, ABCG2, E-Cadherin, CD44, CD90, CD105, CD49a and CD49d in one L-GASC line. In each dot-plot CD133 positive cells are recognized as magenta dots.
stem1605-sup-0006-supptables.docx113KSupporting Information Tables
stem1605-sup-0007-suppinfo.docx193KSupporting Information

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