Neoplastic Reprogramming of Patient-Derived Adipose Stem Cells by Prostate Cancer Cell-Associated Exosomes

Authors

  • Zakaria Y. Abd Elmageed,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Yijun Yang,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Raju Thomas,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Manish Ranjan,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Debasis Mondal,

    1. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Krzysztof Moroz,

    1. Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Department of Pathology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Zhide Fang,

    1. Biostatistics Program, School of Public Health, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
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  • Bashir M. Rezk,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Krishnarao Moparty,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Department of Urology, Southeast Louisiana Veterans Health Care System, New Orleans, Louisiana, USA
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  • Suresh C. Sikka,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Oliver Sartor,

    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
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  • Asim B. Abdel-Mageed

    Corresponding author
    1. Department of Urology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    2. Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    3. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    • *Correspondence: Asim B. Abdel-Mageed, D.V.M., Ph.D., Department of Urology, SL-42, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA. Telephone: +1-504-988-3634; Fax: +1-504-988-5059; e-mail: amageed@tulane.edu

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Abstract

Emerging evidence suggests that mesenchymal stem cells (MSCs) are often recruited to tumor sites but their functional significance in tumor growth and disease progression remains elusive. Herein we report that prostate cancer (PC) cell microenvironment subverts PC patient adipose-derived stem cells (pASCs) to undergo neoplastic transformation. Unlike normal ASCs, the pASCs primed with PC cell conditioned media (CM) formed prostate-like neoplastic lesions in vivo and reproduced aggressive tumors in secondary recipients. The pASC tumors acquired cytogenetic aberrations and mesenchymal-to-epithelial transition and expressed epithelial, neoplastic, and vasculogenic markers reminiscent of molecular features of PC tumor xenografts. Our mechanistic studies revealed that PC cell-derived exosomes are sufficient to recapitulate formation of prostate tumorigenic mimicry generated by CM-primed pASCs in vivo. In addition to downregulation of the large tumor suppressor homolog2 and the programmed cell death protein 4, a neoplastic transformation inhibitor, the tumorigenic reprogramming of pASCs was associated with trafficking by PC cell-derived exosomes of oncogenic factors, including H-ras and K-ras transcripts, oncomiRNAs miR-125b, miR-130b, and miR-155 as well as the Ras superfamily of GTPases Rab1a, Rab1b, and Rab11a. Our findings implicate a new role for PC cell-derived exosomes in clonal expansion of tumors through neoplastic reprogramming of tumor tropic ASCs in cancer patients. Stem Cells 2014;32:983–997

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