• Cell transplantation;
  • Cellular therapy;
  • Clinical translation;
  • Mesenchymal stem cells;
  • Neural stem cell;
  • Progenitor cells;
  • Stem cell transplantation;
  • Transplantation


  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Amyotrophic lateral sclerosis (ALS) is a lethal disease involving the loss of motor neurons. Although the mechanisms responsible for motor neuron degeneration in ALS remain elusive, the development of stem cell-based therapies for the treatment of ALS has gained widespread support. Here, we review the types of stem cells being considered for therapeutic applications in ALS, and emphasize recent preclinical advances that provide supportive rationale for clinical translation. We also discuss early trials from around the world translating cellular therapies to ALS patients, and offer important considerations for future clinical trial design. Although clinical translation is still in its infancy, and additional insight into the mechanisms underlying therapeutic efficacy and the establishment of long-term safety are required, these studies represent an important first step toward the development of effective cellular therapies for the treatment of ALS. Stem Cells 2014;32:1099–1109


  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Amyotrophic lateral sclerosis (ALS) is a lethal adult-onset neurodegenerative disorder characterized by the loss of both upper and lower motor neurons. Sporadic and familial forms are clinically and pathologically indistinguishable, with symptoms including muscle weakness and atrophy that present in either bulbar muscles and/or in the limbs. In almost all cases, death occurs within 3–5 years of diagnosis when progressive motor neuron degeneration affects the diaphragm and leads to respiratory failure. Riluzole, the only FDA-approved treatment for ALS, extends survival for merely a few months [1], highlighting the need for more effective therapies.

The development of targeted therapies for ALS, however, has been hindered by the fact that the mechanisms responsible for disease onset and progression largely remain unknown. Only 10%–15% of cases of ALS are familial, while the remaining 85%–90% of cases are classified as sporadic. Several genetic mutations are associated with familial ALS, including mutations in Cu2+/Zn2+ superoxide dismutase (SOD1) and TAR DNA binding protein-43 [2-5]. Most recently, hexanucleotide repeat expansions in the 5′ noncoding region of the C9orf72 gene have been identified as the most common cause of familial ALS [6]. Among the many proposed mechanisms for the more common sporadic form of ALS [4, 7, 8], oxidative stress and glutamate toxicity induce a toxic cellular and spinal cord milieu, respectively, while neurofilament aggregation and axonal transport defects may be associated with altered mitochondrial trafficking and impaired retrograde transport of peripherally derived neurotrophic factors [4, 8-10]. Recent evidence has also linked protein aggregation and impaired RNA processing to ALS pathogenesis [11, 12]. Furthermore, altered peripheral immunological responses and neuroinflammation are emerging as important effectors of the ALS disease course [13, 14]. Non-neuronal cells such as microglia, astrocytes, and oligodendrocytes also contribute to ALS pathogenesis, via alterations to the spinal cord microenvironment, increased glutamate excitotoxicity, and/or impaired neuronal metabolic support [2, 15-17]. In fact, oligodendrocyte dysfunction is evident early in the disease course before symptom onset [18]. Similarly, denervation at neuromuscular junctions and axonal defects precede symptom onset and motor neuron loss, and studies have shown that connectivity along the entire motor axis, from the corticospinal tract to motor neurons and neuromuscular junctions, is compromised in ALS [19-21]. Therefore, treatments that influence multiple pathogenic mechanisms in ALS and that provide motor neuron support as well as maintain neuronal circuitry are likely required to have the most significant impact on the disease course.

Because of the multifaceted nature of ALS, the emerging concept of stem cell-based therapeutics for ALS treatment has garnered increasing support [22-24]. In this review, we discuss the types of stem cells being considered for therapeutic applications in ALS, highlighting preclinical data supporting the rationale behind the potential efficacy of each cell class and treatment approach. We also discuss some of the early translational studies providing stem cell-based therapies to ALS patients around the world. Although clinical translation is still in its infancy and additional insights into the mechanisms underlying efficacy and the establishment of long-term safety are required, these studies represent an important first step toward the development of cellular therapies for the treatment of ALS.

Preclinical Advances in Stem Cell Therapies for ALS

  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Preclinical in vitro and in vivo studies have provided tremendous insight into which types of stem cells are likely to offer therapeutic benefits in ALS [22, 23]. These lines vary in their derivation source, differentiation potential, and availability; features that all contribute to the advantages and limitations of each population. Understanding how stem cells may confer benefit is also of utmost importance, as transplanted cells can offer cell replacement, provide support through paracrine effects and growth factor production, or alter the immune response and inflammation through cytokine production. Furthermore, treatments aimed at activating endogenous stem cell niches provide a therapeutic option to enhance natural neuroprotective mechanisms. Thus, determining the desired outcomes of stem cell-based therapies is critical for continued therapeutic development. Finally, therapeutic delivery approaches vary and selection of the optimal strategy to achieve the desired effects on ALS pathogenesis warrants important consideration.

Embryonic Stem Cells

Embryonic stem cells (ESCs) have the ability to differentiate into all germ layers, and serve as a resource for both cellular replacement in ALS and for disease modeling when differentiated into motor neurons. Early studies examining intraspinal transplantation of ESC-derived motor neurons into G93A-SOD1 rats demonstrated transient functional improvements; however, there was no apparent axonal projections to the periphery, no effect on neuromuscular junction formation, no long-term effects on the lifespan of the rats, and limited graft survival [25]. Considering the fact that transplanted cells must project axons over long distances in the context of a toxic spinal cord, these results are not surprising and support the contention that direct motor neuron replacement is unlikely to affect the disease course in ALS [25]. Furthermore, their limited supply as well as the fact that human ESCs are subject to strict regulatory policies has hampered continued ESC-based therapeutic advances for ALS; however, the utilization of ESCs for high-throughput drug screening continues, and prospects for future drug development efforts have already been identified using such strategies [26].

Mesenchymal Stem Cells

The therapeutic development of many other stem cell types for clinical application in ALS, conversely, is gaining momentum. Umbilical cord stem cells (UBCs) are harvested from umbilical cord blood and provide a source of mesenchymal stem cells (MSCs) capable of differentiating into mesenchymal and potentially even neuronal lineages [27]. In both G93A-SOD1 and wobbler mice, intracerebroventricular injection of UBCs attenuated progression, and the treatment improved survival by approximately 10% in G93A-SOD1 mice; however, grafted cells were identified within the ventricles and not the spinal cord, suggesting that the observed effects were mediated by production and release of neuroprotective factors, including anti-inflammatory cytokines and chemokines [28]. Similarly, retro-orbital injection of genetically engineered UBCs expressing increased levels of vascular endothelial growth factor (VEGF) and fibroblast growth factor in G93A-SOD1 mice prompted differentiation to astrocytic lineages that produce protective growth factors to improve the motor neuron microenvironment [29]. Retro-orbital delivery of UBCs improved neuromuscular transmission [30], and intravenous UBC administration delayed disease progression by 15%, induced anti-inflammatory effects, reduced microglial activation, and increased survival up to 20%–25% in G93A-SOD1 mice [31, 32]. Intraspinal transplantation of UBCs at an early stage in the disease course improved motor function, attenuated motor neuron loss, reduced astrogliosis, and improved survival by up to 12 days in female G93A-SOD1 mice [33], further supporting the potential of UBC-based therapies in ALS.

Multiple approaches using bone marrow-derived MSCs are also being developed for clinical translation based on the relative accessibility and abundance of MSCs compared to other stem cell classes and their potential for autologous cellular therapy development. Recent reports of MSC cross-lineage differentiation to produce myoblasts and neurons are intriguing, but must be interpreted with caution as this is a relatively new finding and further study is required [24, 34-36]. Intravenous, intrathecal, intracerebral, and intraspinal delivery of autologous MSCs in G93A-SOD1 mice confer a range of beneficial effects on the disease course, including improved motor function, attenuated motor neuron loss, and prolonged survival [22, 23]. G93A-SOD1 mice receiving intraspinal MSC transplants exhibit favorable effects on neuroinflammation, astrogliosis, and microgial activation [37]. Furthermore, induction of neural differentiation of MSCs via neurogenin 1 expression enhances MSC homing to the CNS following intravenous administration in ALS mice and is associated with delayed disease onset and improved motor function [38].

An alternative strategy, however, given that MSCs do not naturally differentiate into neural lineages, is the use of MSCs as a vehicle to deliver neuroprotective factors to the CNS. Intracerebroventricular injection of G93A-SOD1 mice with MSCs expressing glucagon-like peptide 1, a peptide with antioxidant properties, confers significant effects on the disease course, including a 15-day delayed onset, a 13-day improvement in survival, and attenuated neuroinflammation, astrocytosis, and microglial activation [39]. Intramuscular injection of MSCs expressing increased levels of glial-derived neurotrophic factor (GDNF) improved motor neuron health and extended survival by 28 days in G93A-SOD1 rats [40]. The caveat, however, with MSCs or any autologous cellular therapy, is that cells harvested from diseased individuals for therapeutic purposes (in this case with ALS) are inherently predisposed to the disease process. This could clearly impact the therapeutic utility of autologous cells. While some studies characterizing the in vitro properties of MSCs from both ALS patients and healthy controls demonstrate that there are no distinct differences in cellular properties or neuronal differentiation [34, 41], other studies have indicated that ALS patient MSCs exhibit reduced neurotrophic factor secretion and decreased migration [42], and the degree of these deficits correlates with poorer prognosis in ALS patients [43]. Impaired neuroprotective capacity has been observed in MSCs from adult G93A-SOD1 rats [44], and the stability and cellular properties, including neurotrophic profile and anti-inflammatory potential, of MSCs from ALS patients also differ between passages in vitro [45]. Together, these studies suggest that MSCs from healthy donors or utilization of optimally passaged MSCs may confer improved efficacy for cellular therapy development over the use of autologous MSCs from an ALS patient.

Progenitor Cells

Neural progenitor cells (NPCs) are emerging as a promising cellular therapy for clinical translation in ALS. In ALS models, motor neuron degeneration triggers endogenous NPC niches in the CNS to proliferate, migrate, and promote neurogenesis in the spinal cord as a natural response to disease [46, 47]; however, the limited number of endogenous NPCs is likely insufficient to combat the toxic, progressive degeneration associated with ALS. Thus, NPC cell lines with robust growth properties and neurogenic potential have been developed [48, 49] and transplantation of NPCs has been extensively studied as an attempt to augment this natural defense mechanism. NPCs have been delivered via intravenous, intrathecal, and most commonly via intraspinal methods to both G93A-SOD1 mouse and rat models, and the effects on the disease course and cell fate have been extensively characterized [22, 23]. Intraspinal grafting of human NPCs in ALS rats conferred improvements in survival of more than 10 days and protective effects on motor neuron number and motor function that are attributed to the observed ability of transplanted NPCs to integrate into the spinal cord, differentiate, and form functional synapses with host motor neurons [50-52]. Recent observations in G93A-SOD1 rats receiving intraspinal NPC injections, however, revealed that although transient effects on motor neuron number and function were observed in the vicinity of the cellular grafts, electrical recordings of motor-evoked potentials reflected impaired transmission along the descending motor tract and limited effects on survival were present, suggesting that attenuating neuronal loss along the entire corticospinal tract is necessary to achieve meaningful effects on disease progression [21]. This is further supported by studies in G93A-SOD1 rats demonstrating that targeting intraspinal NPC transplants to multiple regions of the spinal cord significantly prolongs survival by 17 days [53]. As in MSC studies, the development of enhanced NPC lines expressing increased levels of growth factors such as GDNF and VEGF also confers improvements in motor neuron number and motor function following transplantation [54, 55], suggesting that combination therapies may warrant consideration in the future. Recent insight into potential crosstalk between transplanted NPCs and endogenous progenitor cell niches in the spinal cord supports the possibility that cellular therapy approaches can induce protection by activating endogenous neuronal repair mechanisms as well [56]. Thus, NPC transplantation has the ability to support motor neurons, provide neurotrophic support, enhance endogenous neurorepair mechanisms, and ultimately maintain neurocircuitry and provide meaningful effects on the ALS disease course.

Finally, the multifaceted mechanisms and variety of cell types proposed to contribute to ALS pathogenesis support cellular therapy development using non-neuronal progenitors. Intracerebroventricular injection of skeletal muscle stem cells induces anti-inflammatory cytokine production, improved motor function, and protection of neuromuscular junctions in wobbler mice [57]. Intraspinal transplantation of glial-restricted progenitor cells into the cervical spinal cord of G93A-SOD1 rats, as a means to increase astrocyte numbers in spinal cord regions controlling critical respiratory function, attenuates motor neuron loss, slows respiratory functional declines, and improves survival by approximately 17 days [58]. Olfactory ensheathing stem cells (OESCs), a type of glial cell that assists in axonal regeneration and is currently in use for spinal cord injury, have also demonstrated positive effects on ALS progression in preclinical studies, including evidence of myelination, increased motor neuron numbers, and more than a 6-day increase in survival following intraspinal transplantation in G93A-SOD1 rats [59]. Analysis of oligodendrocytes in ALS patients and G93A-SOD1 mice revealed defects in the maturation and function of newly proliferating progenitors following degeneration of resident oligodendrocytes during the disease course, suggesting that cellular therapies that restore oligodendrocyte function may be beneficial in ALS [18, 60]. In support of this contention, deletion of G93A-SOD1 in oligodendrocytes of ALS mice delayed disease onset and improved survival [18].

Taken together, these and other studies (detailed further in [22, 23, 61]) demonstrate that multiple classes of stem cells have the potential to impact ALS pathogenesis in preclinical settings by improving the motor neuron environment, replacing lost neuronal and non-neuronal cells within the spinal cord, supporting neuromuscular junctions, and/or modulating the immune response (Fig. 1). Additional discernment of how each stem cell type contributes to these effects and how to most effectively target stem cells to achieve these effects will be incredibly important for selecting the appropriate cell type and designing therapeutic delivery approaches for future clinical translation.


Figure 1. Potential mechanisms of stem cell efficacy in amyotrophic lateral sclerosis. In addition to motor neurons, multiple cell types within the motor neuron microenvironment play a role in disease pathogenesis; therefore, the delivery of stem cell-based therapies (blue) has the potential to provide support through many different mechanisms. Within the spinal cord, stem cells that differentiate into neurons (green) can synapse with existing motor neurons to re-establish or maintain neurocircuitry (A) as well as provide neurotrophic support (B). Differentiation of stem cells into non-neuronal cell types (yellow) within the spinal cord microenvironment can also impact disease progression by providing neurotrophic support (B), and attenuating oligodendrocyte dysfunction and mitigating toxicity (C). In the periphery, stem cell transplantation into muscle can provide critical support to maintain functional neuromuscular junctions (D). Finally, the mobilization of endogenous MSCs from the bone marrow into the circulation can also induce immunomodulatory effects that attenuate inflammatory responses within the spinal cord via the production of cytokines and other anti-inflammatory mediators (E). Abbreviation: MSC, mesenchymal stem cell.

Download figure to PowerPoint

Translating Stem Cell Therapies to ALS Patients

  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Although the age of stem cell-based therapeutics is just beginning, a handful of cellular therapy trials for ALS have been completed in different countries around the world. These recent trials are summarized in Table 1 and include vast differences in the number of patients, cell type, delivery method, and outcome measurement strategies; however, each study has the potential to contribute to our current understanding of the safety and feasibility of stem cell therapies for ALS, as these variables provide important considerations for future trial design and clinical application.

Table 1. Clinical applications of stem cell therapies in ALS.
Stem cell typeDelivery methodCountryPatients no.Desired outcomesConclusionsAdditional detailsReference(s)
  1. Abbreviations: ALSFRS, amyotrophic lateral sclerosis functional rating scale; MSC, mesenchymal stem cell; NPC, neural progenitor cell; OESC, olfactory ensheathing stem cell; SPIO, super paramagnetic iron oxide.

OESCIntracranialPeople's Republic of China15 OESCEfficacyBeneficial effects on disease progressionFollow-up for 4 months; seven patients receiving OESCs showed improvements and two stabilized; only one in the control group remained stableHuang et al. [62]
20 control
IntracranialPeople's Republic of China42EfficacyDelayed progression and restoration of functionReport on patients receiving two to five treatments (out of 507 total patients receiving cellular therapy)Chen et al. [63]
Endogenous MSC mobilizationn/aCanada8SafetyApproach is safe and feasiblePilot study; G-CSF treatment mobilized MSCs with no adverse effectsCashman et al. [64]
n/aItaly24SafetyApproach is safe and feasibleEndogenous MSC mobilized by four G-CSF stimulation treatments at 3-month intervals; At 1 year, no increase in disease progression at rates, anti-inflammatory response observedChio et al. [65]
n/aIsrael17 G-CSFEfficacyApproach is safe and feasible; no significant effect on disease progressionEndogenous MSC mobilized by G-CSF stimulation every 3 months for 1 yearNefussy et al. [66]
18 Placebo
Autologous MSCIntraspinalTurkey13SafetyApproach is safe and feasibleCervical (C1-C2) injections; enrolled patients had bulbar symptoms; nine patients demonstrated electroneuromyography improvements and one had stabilized at 1 yearDeda et al. [67]
 IntraspinalItaly9SafetyApproach is safe and feasibleHigh thoracic (T7-T9) injections; no apparent toxicity, transplant-related adverse events, or structural changes; evidence of slowed functional decline in four patients; follow-up of 4 and up to 9 yearsMazzini et al. [68]
 Mazzini et al. [69]
 IntraspinalItaly10SafetyApproach is safe and feasibleHigh thoracic (T4-T6) injections; no apparent toxicity, transplant-related adverse events, or structural changes; follow-up of 2 or up to 5 yearsMazzini et al. [70]
 Mazzini et al. [69]
 IntraspinalSpain11SafetyApproach is safe and feasibleNo acceleration in decline noted; increased motor neuron numbers noted in treated spinal cord segments at autopsy; motor neurons surrounded by CD90+ cells without degenerative ubiquitin depositsBlanquer et al. [71]
 IntrathecalIndia10SafetyApproach is safe and feasible; initial trend toward stabilization of diseaseNo significant acceleration of ALSFRS decline after short-term follow-up at 1 year; confirmation of benefit required with long-term follow-upPrabhakar et al. [72]
 Intrathecal or combined intrathecal/intravenousIsrael10 intrathecalSafetyApproach is safe and feasibleBoth intrathecal and combination intrathecal/intravenous administration approaches are safe; SPIO labeling in a subset of patients revealed cells in meninges, subarachnoid space and spinal cord; immunomodulatory effects observed; follow-up period of 6–25 months with MRI at 1 yearKarussis et al. [73]
 9 combined
 IntraventricularSouth Korea1SafetyApproach is safe and feasibleOmmaya reservoir used to administer three injections at 1 month intervals; no conclusions on efficacy due to advanced disease state of patientBaek et al. [74]
 Motor CortexMexico10 MSCSafetyApproach is safe and feasiblePilot study; procedure is safe and well-tolerated; survival statistically higher in treated patientsMartinez et al. [75]
 10 control
 Motor CortexMexico65SafetyApproach is safe and feasibleTargeted frontal motor cortex to improve upper motor neuron function; larger controlled trial required to assess efficacyMartinez et al. [76]
T-cell vaccination coupled with autologous MSC and NPC therapyIntravenousArgentina7EfficacyApproach is safe and feasible; median survival extendedDual cellular therapy approach; neurological recovery noted in five patientsMoviglia et al. [77]
NPCIntraspinalU.S.12SafetyApproach is safe and feasibleLumbar (L2-L4) injections; follow-up of 6–18 months; no acceleration of disease course present; one patient demonstrated improvements; continued follow-up also availableGlass et al. [78]
Riley et al. [79]
Riley et al. [80]
IntraspinalU.S.6SafetyApproach is safe and feasibleCervical (C3-C5) injections; three patients were part of the initial lumbar cohort (above) and received dual targeting to both lumbar and cervical regionsRiley et al. [80]

Clinical Application of OESCs

OESC transplantation for ALS has commenced in People's Republic of China based on positive effects, including axonal regeneration, remyelination, and functional improvements, in spinal cord injury studies [81]. In a small controlled study involving 35 ALS patients, 15 of which received OESC transplants and 20 untreated controls, individuals receiving intracranial OESC transplants showed decreased progression, as measured by the ALS functional rating scale (ALSFRS), over the 4-month follow-up period [62]. Based on these and other short-term results from this group [82], over 500 ALS patients have since received OESC transplants in People's Republic of China, with the majority of individuals receiving a single intracranial OESC injection, and 42 patients receiving two to five rounds of OESC injections [63]. Evaluation of the 42 patients receiving multiple rounds of OESCs revealed improvements in ALSFRS and ALS Norris Scale scores as well as improvements in neurological and pulmonary function after repeated intervention [63]. Independent follow-up case reports on individuals receiving OESC transplants in People's Republic of China, however, do not support the clinical translation of this OESC therapy. Evaluation of seven patients for 1 year revealed no significant objective improvements and two patients experienced serious side effects [83], and similarly, no benefit was seen in an elderly woman who experienced accelerated disease progression and severe side effects following OESC therapy [84]. Furthermore, post-mortem characterization of the cellular grafts in two Italian patients treated in People's Republic of China exhibited evidence of graft encasement, the presence of undifferentiated cells, and no evidence of neurotrophism or regeneration [85]. Thus, while the large Chinese study reports that OESCs may offer benefit in ALS, other reports criticize the observed outcomes and do not support the clinical translation of this therapeutic approach at this time. Furthermore, these findings strongly emphasize the need for continued research, including additional preclinical validation, detailed graft characterization, and long-term well-designed trials, to support the safety and utility of OESC-based therapies in ALS patients.

Clinical Applications of MSC-Based Therapies for ALS

The largest number of cellular therapy trials for ALS involves MSCs. Three clinical trials have tested strategies using granulocyte colony-stimulating factor (GCSF) to mobilize endogenous MSCs in ALS patients. Trials based in Canada and Italy have demonstrated safety of the approach, confirmed mobilization of MSCs, and demonstrated anti-inflammatory responses in the spinal cord [64, 65]. A pilot study in Israel also demonstrated safety and feasibility of GCSF-induced MSC mobilization; however, no significant effects on disease progression were noted compared to placebo-treated controls [66]. Alternatively, a number of trials assessing autologous MSC treatment approaches around the world have demonstrated the safety and feasibility of intraspinal, intrathecal, and intracerebral MSC transplants [24]. Although safety was the primary question in the majority of these studies, secondary outcome assessment in a trial of 13 patients in Turkey receiving intraspinal C1-C2 MSC transplants demonstrated encouraging effects on electoneuromyography measures in the majority of patients [67], and secondary outcome evaluation of 11 patients receiving intraspinal MSC transplants in Spain revealed increased motor neuron numbers, reductions in the presence of ubiquitin deposits in motor neurons, and evidence of neurotrophism in treated spinal cord segments [71]. In addition, a controlled pilot study in Mexico examining the safety of intracranial MSC delivery in 20 ALS patients reported that survival was significantly extended in treated patients [75]. Together, these trials provide important insight into the safety and feasibility of autologous MSC-based therapies in ALS patients, although determining the efficacy of these approaches requires continued insight into the ability of MSCs to home to the CNS, insight into the mechanisms of neuroprotection, and large, controlled studies to evaluate efficacy.

Clinical Application of NPC-Based Therapies

Given the vast amount of preclinical support for NPC-based therapies, an FDA-approved clinical trial assessing the safety and feasibility of intraspinal injection of human spinal stem cells (HSSCs) in ALS patients is currently in progress in the U.S. [22, 23, 78, 79]. Using a customized injection device designed for safe, reproducible, accurate delivery of biologics to the spinal cord [22, 78, 79, 86-89], 18 intraspinal transplantation surgeries were performed following a risk escalation paradigm to complete phase I of the trial. Twelve patients received L2-L5 lumbar-targeted intraspinal injections and six patients received C3-C5 cervical-targeted intraspinal injections with no major adverse effects attributed to the surgery or cells [78-80]. Of note, the last three patients receiving cervical HSSC transplants had previously received lumbar transplants, demonstrating that targeting multiple levels of the spinal cord is feasible in ALS patients, an approach associated with improved therapeutic efficacy in preclinical studies [53]. Patients were all evaluated for multiple functional measures, including ALSFRS, respiratory function, and muscle strength, and demonstrated no acceleration in progression following transplants, and one patient exhibited improved functional measures; however, phase II of the trial, which began in September 2013, will be required to assess HSSC dosing and efficacy of the intervention.

The Future of Stem Cell Therapies for ALS

  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Overall, multiple groups have assessed the safety of cellular grafting along the entire neural axis, using systemic approaches and targeting brain regions as well as the upper and lower spinal cord, and demonstrated that delivery approaches and the introduction of stem cell populations into these regions can be successfully and safely accomplished. While considerable work is still required, these data provide proof-of-concept that cellular grafting as a therapy for ALS is feasible and support a continued focus on refining stem cell-based therapeutic approaches to achieve maximal benefit in ALS.

As we look to the future, a number of important considerations must still be addressed to support stem cell therapies for the treatment of ALS. First, elucidating the proper approach to deliver or target cellular therapies to regions where it will have maximal benefit in ALS patients is of utmost importance. As detailed in the previous section, strategies ranging from intravenous, intraspinal, and intraventricular injection of cellular therapies to treatments designed to activate or mobilize endogenous progenitor populations are currently being pursued. While there may not be a single ideal approach, establishing standardized practices for the delivery of cellular therapies to ensure reproducible injection volumes and targeting accuracy will assist in the design and interpretation of future clinical trials. For example, the recent development and utilization in a clinical trial of novel devices for intraspinal delivery of cellular therapies is already realizing these objectives [22, 78, 79, 86-89].

Second, confirmation of graft survival is imperative to achieve sustained efficacy. In many preclinical in vivo studies, the identification of grafted human cells in animal models is facilitated by immunohistochemical identification using human-specific markers [50, 52, 86]. Upon clinical translation, however, more sophisticated techniques to identify and follow the fate of grafted cells will be required. For instance, the labeling of cells with superparamagnetic iron oxide nanoparticles or reporter genes allows cell graft tracking using advanced imaging technologies [28, 57, 90-95]. Notably, these approaches can provide insight into the migratory potential of grafted cells following systemic or targeted injections, as demonstrated in both a large animal and human trials [73, 86]. Understanding how grafted cells migrate into or within the spinal cord will be necessary to optimize delivery approaches and maximally impact critical cellular populations, including both upper and lower motor neurons, and maintain functional neurocircuitry along the entire corticospinal tract.

Third, requirements for immunosuppression must also be determined. Graft survival of transplanted human NPCs is enhanced using combined immunosuppression regimens in ALS models and in a large mammal, the Gottingen minipig [86, 96]. What is now required are data from human trials to determine the role of the immune system and immunosuppression requirements when transplanting human cells into ALS patients. Additional longitudinal data from ongoing and future clinical trials will provide important insight into graft survival, characterization, and treatment efficacy.

As we continue to move forward and optimize how to best deliver cellular therapies, emphasis on the design of future clinical trials will also be necessary to glean meaningful insight into the safety and efficacy of clinical outcomes [97, 98]. Common outcome measures in these ALS trials include ALSFRS assessment and other functional measures as well as survival; however, the elucidation and inclusion of novel prognostic biomarkers in ALS trials may provide additional power and inform patient selection criteria [97, 98]. Given the heterogeneous presentation and rapid progression of ALS, consideration of limb versus bulbar onset ALS and early versus late disease, and knowledge of disease progression rates prior to cellular delivery, may be required patient selection criteria in future trials.

Finally, novel cellular therapy development may be possible with continued advances in the field of stem cell research. Since the initial reports generating induced pluripotent stem (iPS) cells from somatic tissue, established differentiation protocols have enabled the development of patient-specific iPS-derived motor neurons from ALS patients for in vitro characterization and drug screening [99-102]. These cells may also offer an autologous source for cellular therapy that circumvents the need for immunosuppression; however, the clinical application of iPS cellular therapies has not yet been attempted or realized, and insight into how the inherent predisposition to disease these cells may possess affects their therapeutic potential is required. Nonetheless, constant protocol refinements, such as alternative methods to introduce genetic reprogramming factors and the most recent reports of iPS cell generation using a chemical-based approach, are supporting the potential for future preclinical and clinical therapeutic applications of this technology [103-107]. Continued development of enhanced stem cell lines, such as those expressing increased levels of neurotrophic growth factors, may also gain ground in future translational studies, as this approach has the potential to combine the benefits of growth factor delivery in ALS with cellular support offered by stem cell-based therapies and form a multifaceted attack on ALS [108, 109]. In addition, cellular therapy approaches combining NPC, glial progenitor cell, and/or skeletal muscle cell treatment modalities may offer additive benefit against pathogenenic mechanisms conferred at the level of neurons, glia, and in muscle at peripheral neuromuscular junctions. Indications for the future combination of immune modulation with stem cell therapies as a possible therapeutic avenue also exist, as an Argentinian clinical trial combining MSC or NPC transplantation with T-cell vaccination demonstrated safety, improvements in median survival, and evidence of neurological recovery in five out of seven patients [77]. Overall, ensuring that cellular therapies are capable of providing long-term benefits that affect motor neurons, their environment, and connectivity along the entire neuroaxis is likely critical to achieve meaningful outcomes in ALS.


  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

Although much work remains to be done, the increasing focus on preclinical research for stem cell therapies and the recent translation of a small number of these therapies to clinical trials have set the stage for continued progress. In the near future, efforts must continue to determine the most efficacious cell type and identify appropriate approaches to safely administer cellular therapies to achieve positive outcomes in ALS. With the establishment of best practice guidelines for cellular therapies, it may then be possible for future endeavors to address strategies that use novel cellular sources, engineer enhanced stem cells, or develop combinatorial therapeutic approaches in order to provide potentially meaningful therapies for this lethal disorder.


  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

We would like to thank Judith Bentley for excellent administrative support during the preparation of this manuscript. This work was supported by the National Institutes of Health (R01 NS077982), the A. Alfred Taubman Medical Research Institute, the Program for Neurology Research & Discovery, the Katherine Rayner Fund, the Cox Foundation, and the Virginia Gentlemen Foundation.

Author Contributions

  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References

J.S.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, and final approval of manuscript; S.A.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript; E.L.F.: conception and design, financial support, manuscript writing, and final approval of manuscript. J.S.L. and S.A.S. contributed equally to this article.


  1. Top of page
  2. Abstract
  3. Introduction
  4. Preclinical Advances in Stem Cell Therapies for ALS
  5. Translating Stem Cell Therapies to ALS Patients
  6. The Future of Stem Cell Therapies for ALS
  7. Conclusions
  8. Acknowledgments
  9. Author Contributions
  10. Disclosure of Potential Conflicts of Interest
  11. References
  • 1
    Borasio G, Miller R. Clinical characteristics and management of ALS. Semin Neurosci 2001;21:155166.
  • 2
    Ilieva H, Polymenidou M, Cleveland DW. Non-cell autonomous toxicity in neurodegenerative disorders: ALS and beyond. J Cell Biol 2009;187:761772.
  • 3
    Kabashi E, Valdmanis PN, Dion P et al. TARDBP mutations in individuals with sporadic and familial amyotrophic lateral sclerosis. Nat Genet 2008;40:572574.
  • 4
    Rothstein JD. Current hypotheses for the underlying biology of amyotrophic lateral sclerosis. Ann Neurol 2009;65(suppl 1):S39.
  • 5
    Sreedharan J, Blair IP, Tripathi VB et al. TDP-43 mutations in familial and sporadic amyotrophic lateral sclerosis. Science 2008;319:16681672.
  • 6
    van Blitterswijk M, DeJesus-Hernandez M, Rademakers R. How do C9ORF72 repeat expansions cause amyotrophic lateral sclerosis and frontotemporal dementia: Can we learn from other noncoding repeat expansion disorders? Curr Opin Neurol 2012;25:689700.
  • 7
    Bruijn LI, Miller TM, Cleveland DW. Unraveling the mechanisms involved in motor neuron degeneration in ALS. Annu Rev Neurosci 2004;27:723749.
  • 8
    Gordon PH. Amyotrophic Lateral Sclerosis: An update for 2013 clinical features, pathophysiology, management and therapeutic trials. Aging Dis 2013;4:295310.
  • 9
    Cozzolino M, Pesaresi MG, Gerbino V et al. Amyotrophic lateral sclerosis: New insights into underlying molecular mechanisms and opportunities for therapeutic intervention. Antioxid Redox Signal 2012;17:12771330.
  • 10
    Pizzuti A, Petrucci S. Mitochondrial disfunction as a cause of ALS. Arch Ital Biol 2011;149:113119.
  • 11
    Polymenidou M, Lagier-Tourenne C, Hutt KR et al. Misregulated RNA processing in amyotrophic lateral sclerosis. Brain Res 2012;1462:315.
  • 12
    Ticozzi N, Ratti A, Silani V. Protein aggregation and defective RNA metabolism as mechanisms for motor neuron damage. CNS Neurol Disord Drug Targets 2010;9:285296.
  • 13
    Evans MC, Couch Y, Sibson N et al. Inflammation and neurovascular changes in amyotrophic lateral sclerosis. Mol Cell Neurosci 2013;53:3441.
  • 14
    Rizzo F, Riboldi G, Salani S et al. Cellular therapy to target neuroinflammation in amyotrophic lateral sclerosis. Cell Mol Life Sci 2013 [Epub ahead of print], DOI 10.1007/s00018-013-1480-4. (PMID 24100629).
  • 15
    Lee Y, Morrison BM, Li Y et al. Oligodendroglia metabolically support axons and contribute to neurodegeneration. Nature 2012;487:443448.
  • 16
    Boillee S, Vande Velde C, Cleveland DW. ALS: A disease of motor neurons and their nonneuronal neighbors. Neuron 2006;52:3959.
  • 17
    Lasiene J, Yamanaka K. Glial cells in amyotrophic lateral sclerosis. Neurol Res Int 2011;2011:718987.
  • 18
    Kang SH, Li Y, Fukaya M et al. Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis. Nat Neurosci 2013;16:571579.
  • 19
    Fischer LR, Culver DG, Tennant P et al. Amyotrophic lateral sclerosis is a distal axonopathy: Evidence in mice and man. Exp Neurol 2004;185:232240.
  • 20
    Fischer LR, Glass JD. Axonal degeneration in motor neuron disease. Neurodegener Dis 2007;4:431442.
  • 21
    Hefferan MP, Galik J, Kakinohana O et al. Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation. PLoS One 2012;7:e42614.
  • 22
    Boulis NM, Federici T, Glass JD et al. Translational stem cell therapy for amyotrophic lateral sclerosis. Nat Rev Neurol 2011;8:172176.
  • 23
    Lunn JS, Sakowski SA, Federici T et al. Stem cell technology for the study and treatment of motor neuron diseases. Regen Med 2011;6:201213.
  • 24
    Minguell JJ, Allers C, Lasala GP. Mesenchymal stem cells and the treatment of conditions and diseases: The less glittering side of a conspicuous stem cell for basic research. Stem Cells Dev 2013;22:193203.
  • 25
    Lopez-Gonzalez R, Kunckles P, Velasco I. Transient recovery in a rat model of familial amyotrophic lateral sclerosis after transplantation of motor neurons derived from mouse embryonic stem cells. Cell Transplant 2009;18:11711181.
  • 26
    Yang YM, Gupta SK, Kim KJ et al. A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS. Cell Stem Cell 2013;12:713726.
  • 27
    Divya MS, Roshin GE, Divya TS et al. Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation. Stem Cell Res Ther 2012;3:57.
  • 28
    Bigini P, Veglianese P, Andriolo G et al. Intracerebroventricular administration of human umbilical cord blood cells delays disease progression in two murine models of motor neuron degeneration. Rejuvenation Res 2011;14:623639.
  • 29
    Rizvanov AA, Guseva DS, Salafutdinov, II et al. Genetically modified human umbilical cord blood cells expressing vascular endothelial growth factor and fibroblast growth factor 2 differentiate into glial cells after transplantation into amyotrophic lateral sclerosis transgenic mice. Exp Biol Med (Maywood) 2011;236:9198.
  • 30
    Souayah N, Coakley KM, Chen R et al. Defective neuromuscular transmission in the SOD1 G93A transgenic mouse improves after administration of human umbilical cord blood cells. Stem Cell Rev 2012;8:224228.
  • 31
    Garbuzova-Davis S, Sanberg CD, Kuzmin-Nichols N et al. Human umbilical cord blood treatment in a mouse model of ALS: Optimization of cell dose. PLoS One 2008;3:e2494.
  • 32
    Garbuzova-Davis S, Willing AE, Zigova T et al. Intravenous administration of human umbilical cord blood cells in a mouse model of amyotrophic lateral sclerosis: Distribution, migration, and differentiation. J Hematother Stem Cell Res 2003;12:255270.
  • 33
    Knippenberg S, Thau N, Schwabe K et al. Intraspinal injection of human umbilical cord blood-derived cells is neuroprotective in a transgenic mouse model of amyotrophic lateral sclerosis. Neurodegener Dis 2012;9:107120.
  • 34
    Gonzalez-Garza MT, Martinez HR, Caro-Osorio E et al. Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells. Stem Cells Transl Med 2013;2:129135.
  • 35
    Jiang Y, Vaessen B, Lenvik T et al. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol 2002;30:896904.
  • 36
    Singh SP, Tripathy NK, Nityanand S. Comparison of phenotypic markers and neural differentiation potential of multipotent adult progenitor cells and mesenchymal stem cells. World J Stem Cells 2013;5:5360.
  • 37
    Vercelli A, Mereuta OM, Garbossa D et al. Human mesenchymal stem cell transplantation extends survival, improves motor performance and decreases neuroinflammation in mouse model of amyotrophic lateral sclerosis. Neurobiol Dis 2008;31:395405.
  • 38
    Chan-II C, Young-Don L, Heejaung K et al. Neural Induction with Neurogenin 1 Enhances the Therapeutic Potential of Mesenchymal Stem Cells in an ALS Mouse Model. Cell Transplant 2013;22:855870.
  • 39
    Knippenberg S, Thau N, Dengler R et al. Intracerebroventricular injection of encapsulated human mesenchymal cells producing glucagon-like peptide 1 prolongs survival in a mouse model of ALS. PLoS One 2012;7:e36857.
  • 40
    Suzuki M, McHugh J, Tork C et al. Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS. Mol Ther 2008;16:20022010.
  • 41
    Ferrero I, Mazzini L, Rustichelli D et al. Bone marrow mesenchymal stem cells from healthy donors and sporadic amyotrophic lateral sclerosis patients. Cell Transplant 2008;17:255266.
  • 42
    Cho GW, Noh MY, Kim HY et al. Bone marrow-derived stromal cells from amyotrophic lateral sclerosis patients have diminished stem cell capacity. Stem Cells Dev 2010;19:10351042.
  • 43
    Koh SH, Baik W, Noh MY et al. The functional deficiency of bone marrow mesenchymal stromal cells in ALS patients is proportional to disease progression rate. Exp Neurol 2012;233:472480.
  • 44
    Boucherie C, Caumont AS, Maloteaux JM et al. In vitro evidence for impaired neuroprotective capacities of adult mesenchymal stem cells derived from a rat model of familial amyotrophic lateral sclerosis (hSOD1(G93A)). Exp Neurol 2008;212:557561.
  • 45
    Choi MR, Kim HY, Park JY et al. Selection of optimal passage of bone marrow-derived mesenchymal stem cells for stem cell therapy in patients with amyotrophic lateral sclerosis. Neurosci Lett 2010;472:9498.
  • 46
    Chi L, Ke Y, Luo C et al. Motor neuron degeneration promotes neural progenitor cell proliferation, migration, and neurogenesis in the spinal cords of amyotrophic lateral sclerosis mice. Stem Cells 2006;24:3443.
  • 47
    Juan L, Dawei Z, Julie AD. Increased number and differentiation of neural precursor cells in the brainstem of superoxide dismutase 1(G93A) (G1H) transgenic mouse model of amyotrophic lateral sclerosis. Neurol Res 2007;29:204209.
  • 48
    Gelati M, Profico D, Projetti-Pensi M et al. Culturing and expansion of “clinical grade” precursors cells from the fetal human central nervous system. Methods Mol Biol 2013;1059:6577.
  • 49
    Guo X, Johe K, Molnar P et al. Characterization of a human fetal spinal cord stem cell line, NSI-566RSC, and its induction to functional motoneurons. J Tissue Eng Regen Med 2010;4:181193.
  • 50
    Xu L, Ryugo DK, Pongstaporn T et al. Human neural stem cell grafts in the spinal cord of SOD1 transgenic rats: Differentiation and structural integration into the segmental motor circuitry. J Comp Neurol 2009;514:297309.
  • 51
    Xu L, Yan J, Chen D et al. Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats. Transplantation 2006;82:865875.
  • 52
    Yan J, Xu L, Welsh AM et al. Extensive neuronal differentiation of human neural stem cell grafts in adult rat spinal cord. PLoS Med 2007;4:e39.
  • 53
    Xu L, Shen P, Hazel T et al. Dual transplantation of human neural stem cells into cervical and lumbar cord ameliorates motor neuron disease in SOD1 transgenic rats. Neurosci Lett 2011;494:222226.
  • 54
    Hwang DH, Lee HJ, Park IH et al. Intrathecal transplantation of human neural stem cells overexpressing VEGF provide behavioral improvement, disease onset delay and survival extension in transgenic ALS mice. Gene Ther 2009;16:12341244.
  • 55
    Suzuki M, McHugh J, Tork C et al. GDNF secreting human neural progenitor cells protect dying motor neurons, but not their projection to muscle, in a rat model of familial ALS. PLoS One 2007;2:e689.
  • 56
    Xu L, Mahairaki V, Koliatsos VE. Host induction by transplanted neural stem cells in the spinal cord: Further evidence for an adult spinal cord neurogenic niche. Regen Med 2012;7:785797.
  • 57
    Canzi L, Castellaneta V, Navone S et al. Human skeletal muscle stem cell antiinflammatory activity ameliorates clinical outcome in amyotrophic lateral sclerosis models. Mol Med 2012;18:401411.
  • 58
    Lepore AC, Rauck B, Dejea C et al. Focal transplantation-based astrocyte replacement is neuroprotective in a model of motor neuron disease. Nat Neurosci 2008;11:12941301.
  • 59
    Li Y, Bao J, Khatibi NH et al. Olfactory ensheathing cell transplantation into spinal cord prolongs the survival of mutant SOD1(G93A) ALS rats through neuroprotection and remyelination. Anat Rec (Hoboken) 2011;294:847857.
  • 60
    Philips T, Bento-Abreu A, Nonneman A et al. Oligodendrocyte dysfunction in the pathogenesis of amyotrophic lateral sclerosis. Brain 2013;136:471482.
  • 61
    Meamar R, Nasr-Esfahani MH, Mousavi SA et al. Stem cell therapy in amyotrophic lateral sclerosis. J Clin Neurosci 2013;20:16591663.
  • 62
    Huang H, Chen L, Xi H et al. Fetal olfactory ensheathing cells transplantation in amyotrophic lateral sclerosis patients: A controlled pilot study. Clin Transplant 2008;22:710718.
  • 63
    Chen L, Chen D, Xi H et al. Olfactory ensheathing cell neurorestorotherapy for amyotrophic lateral sclerosis patients: Benefits from multiple transplantations. Cell Transplant 2012;21(suppl 1):S6577.
  • 64
    Cashman N, Tan LY, Krieger C et al. Pilot study of granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood stem cells in amyotrophic lateral sclerosis (ALS). Muscle Nerve 2008;37:620625.
  • 65
    Chio A, Mora G, La Bella V et al. Repeated courses of granulocyte colony-stimulating factor in amyotrophic lateral sclerosis: Clinical and biological results from a prospective multicenter study. Muscle Nerve 2011;43:189195.
  • 66
    Nefussy B, Artamonov I, Deutsch V et al. Recombinant human granulocyte-colony stimulating factor administration for treating amyotrophic lateral sclerosis: A pilot study. Amyotroph Lateral Scler 2010;11:187193.
  • 67
    Deda H, Inci MC, Kurekci AE et al. Treatment of amyotrophic lateral sclerosis patients by autologous bone marrow-derived hematopoietic stem cell transplantation: A 1-year follow-up. Cytotherapy 2009;11:1825.
  • 68
    Mazzini L, Mareschi K, Ferrero I et al. Stem cell treatment in amyotrophic lateral sclerosis. J Neurol Sci 2008;265:7883.
  • 69
    Mazzini L, Mareschi K, Ferrero I et al. Mesenchymal stromal cell transplantation in amyotrophic lateral sclerosis: A long-term safety study. Cytotherapy 2012;14:5660.
  • 70
    Mazzini L, Ferrero I, Luparello V et al. Mesenchymal stem cell transplantation in amyotrophic lateral sclerosis: A phase I clinical trial. Exp Neurol 2010;223:229237.
  • 71
    Blanquer M, Moraleda JM, Iniesta F et al. Neurotrophic bone marrow cellular nests prevent spinal motoneuron degeneration in amyotrophic lateral sclerosis patients: A pilot safety study. Stem Cells 2012;30:12771285.
  • 72
    Prabhakar S, Marwaha N, Lal V et al. Autologous bone marrow-derived stem cells in amyotrophic lateral sclerosis: A pilot study. Neurol India 2012;60:465469.
  • 73
    Karussis D, Karageorgiou C, Vaknin-Dembinsky A et al. Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis. Arch Neurol 2010;67:11871194.
  • 74
    Baek W, Kim YS, Koh SH et al. Stem cell transplantation into the intraventricular space via an Ommaya reservoir in a patient with amyotrophic lateral sclerosis. J Neurosurg Sci 2012;56:261263.
  • 75
    Martinez HR, Gonzalez-Garza MT, Moreno-Cuevas JE et al. Stem-cell transplantation into the frontal motor cortex in amyotrophic lateral sclerosis patients. Cytotherapy 2009;11:2634.
  • 76
    Martinez HR, Molina-Lopez JF, Alez-Garza MT et al. Stem cell transplantation in amyotrophic lateral sclerosis patients. Methodological approach, safety, and feasibility. Cell Transplant 2012;21:18991907.
  • 77
    Moviglia GA, Moviglia-Brandolino MT, Varela GS et al. Feasibility, safety, and preliminary proof of principles of autologous neural stem cell treatment combined with T-cell vaccination for ALS patients. Cell Transplant 2012;21(suppl 1):S5763.
  • 78
    Glass JD, Boulis NM, Johe K et al. Lumbar intraspinal injection of neural stem cells in patients with amyotrophic lateral sclerosis: Results of a phase I trial in 12 patients. Stem Cells 2012;30:11441151.
  • 79
    Riley J, Federici T, Polak M et al. Intraspinal stem cell transplantation in amyotrophic lateral sclerosis: A phase I safety trial, technical note, and lumbar safety outcomes. Neurosurgery 2012;71:405416.
  • 80
    Riley J, Glass J, Feldman EL et al. Intraspinal stem cell transplantation in ALS: A phase I trial, cervical microinjection and final surgical safety outcomes. Neurosurgery 2014;74:7787.
  • 81
    Huang H, Chen L, Wang H et al. Influence of patients' age on functional recovery after transplantation of olfactory ensheathing cells into injured spinal cord injury. Chin Med J (Engl) 2003;116:14881491.
  • 82
    Chen L, Huang H, Zhang J et al. Short-term outcome of olfactory ensheathing cells transplantation for treatment of amyotrophic lateral sclerosis. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2007;21:961966.
  • 83
    Piepers S, van den Berg LH. No benefits from experimental treatment with olfactory ensheathing cells in patients with ALS. Amyotroph Lateral Scler 2010;11:328330.
  • 84
    Chew S, Khandji AG, Montes J et al. Olfactory ensheathing glia injections in Beijing: Misleading patients with ALS. Amyotroph Lateral Scler 2007;8:314316.
  • 85
    Giordana MT, Grifoni S, Votta B et al. Neuropathology of olfactory ensheathing cell transplantation into the brain of two amyotrophic lateral sclerosis (ALS) patients. Brain Pathol 2010;20:730737.
  • 86
    Raore B, Federici T, Taub J et al. Cervical multilevel intraspinal stem cell therapy: Assessment of surgical risks in Gottingen minipigs. Spine (Phila Pa 1976) 2011;36:E164171.
  • 87
    Riley J, Butler J, Park J et al. Targeted spinal cord therapeutics delivery: Stabilized platform and MER guidance validation. Stereotact Funct Neurosurg 2007;86:6774.
  • 88
    Riley J, Federici T, Park J et al. Cervical spinal cord therapeutics delivery: Preclinical safety validation of a stabilized microinjection platform. Neurosurgery 2009;65:754761; discussion 761-752.
  • 89
    Riley JP, Raore B, Taub JS et al. Platform and cannula design improvements for spinal cord therapeutics delivery. Neurosurgery 2011;69:147154.
  • 90
    Wang F, Dennis JE, Awadallah A et al. Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging. Physiol Genomics 2009;37:2334.
  • 91
    Yaghoubi SS, Campbell DO, Radu CG et al. Positron emission tomography reporter genes and reporter probes: Gene and cell therapy applications. Theranostics 2012;2:374391.
  • 92
    Zhang SJ, Wu JC. Comparison of imaging techniques for tracking cardiac stem cell therapy. J Nucl Med 2007;48:19161919.
  • 93
    Lamanna JJ, Donnelly EM, Oshinski JN et al. 177 pre-clinical validation of superparamagnetic iron oxide nanoparticle-labeled neural stem cells for in vivo tracking and post-mortem identification in the spinal cord. Neurosurgery 2012;71:E569.
  • 94
    Neri M, Maderna C, Cavazzin C et al. Efficient in vitro labeling of human neural precursor cells with superparamagnetic iron oxide particles: Relevance for in vivo cell tracking. Stem Cells 2008;26:505516.
  • 95
    Hu SL, Zhang JQ, Hu X et al. In vitro labeling of human umbilical cord mesenchymal stem cells with superparamagnetic iron oxide nanoparticles. J Cell Biochem 2009;108:529535.
  • 96
    Hefferan MP, Johe K, Feldman EL et al. Optimization of immunosuppressive therapy for spinal grafting of human spinal stem cells in a rat model of ALS. Cell Transplant 2011;20:11531161.
  • 97
    Gladman M, Cudkowicz M, Zinman L. Enhancing clinical trials in neurodegenerative disorders: Lessons from amyotrophic lateral sclerosis. Curr Opin Neurol 2012;25:735742.
  • 98
    Healy BC, Schoenfeld D. Comparison of analysis approaches for phase III clinical trials in amyotrophic lateral sclerosis. Muscle Nerve 2012;46:506511.
  • 99
    Dimos JT, Rodolfa KT, Niakan KK et al. Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 2008;321:12181221.
  • 100
    Egawa N, Kitaoka S, Tsukita K et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 2012;4:145ra104.
  • 101
    Luo Y, Fan Y, Chen X et al. Generation of induced pluripotent stem cells from asian patients with chronic neurodegenerative diseases. J Reprod Dev 2012;58:515521.
  • 102
    Yamanaka S. Induction of pluripotent stem cells from mouse fibroblasts by four transcription factors. Cell Prolif 2008;41(suppl 1):5156.
  • 103
    Hou P, Li Y, Zhang X et al. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 2013;341:651654.
  • 104
    Cho HJ, Lee CS, Kwon YW et al. Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation. Blood 2010;116:386395.
  • 105
    Yakubov E, Rechavi G, Rozenblatt S et al. Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors. Biochem Biophys Res Commun 2010;394:189193.
  • 106
    Yamanaka S, Blau HM. Nuclear reprogramming to a pluripotent state by three approaches. Nature 2010;465:704712.
  • 107
    O'Doherty R, Greiser U, Wang W. Nonviral methods for inducing pluripotency to cells. Biomed Res Int 2013;2013:705902.
  • 108
    Lunn JS, Hefferan MP, Marsala M et al. Stem cells: Comprehensive treatments for amyotrophic lateral sclerosis in conjunction with growth factor delivery. Growth Factors 2009;27:133140.
  • 109
    Suzuki M, Svendsen CN. Combining growth factor and stem cell therapy for amyotrophic lateral sclerosis. Trends Neurosci 2008;31:192198.