Data access: The mRNA-seq datasets can be accessed via GEO (http://www.ncbi.nlm.nih.gov/geo) accession number GSE53297.
Tissue-Specific Stem Cells
Adult Stem Cells in the Small Intestine Are Intrinsically Programmed with Their Location-Specific Function
Version of Record online: 17 APR 2014
© 2014 AlphaMed Press
Volume 32, Issue 5, pages 1083–1091, May 2014
How to Cite
Middendorp, S., Schneeberger, K., Wiegerinck, C. L., Mokry, M., Akkerman, R. D. L., van Wijngaarden, S., Clevers, H. and Nieuwenhuis, E. E. S. (2014), Adult Stem Cells in the Small Intestine Are Intrinsically Programmed with Their Location-Specific Function. STEM CELLS, 32: 1083–1091. doi: 10.1002/stem.1655
- Issue online: 17 APR 2014
- Version of Record online: 17 APR 2014
- Accepted manuscript online: 4 FEB 2014 10:06AM EST
- Manuscript Accepted: 28 DEC 2013
- Manuscript Revised: 21 DEC 2013
- Manuscript Received: 22 JUL 2013
Additional Supporting Information may be found in the online version of this article.
|stem1655-sup-0001-supptbl1.doc||38K||Table S1. Real time RT-PCR primer sequences|
|stem1655-sup-0002-supptbl2.doc||252K||Table S2. Gene ontology of Biological Processes for location-specific crypt genes|
|stem1655-sup-0003-suppfig1.tif||16502K||Supplementary Figure 1. Addition of WNT3A to murine organoid cultures induces proliferation and inhibits differentiation. Murine organoids derived from duodenum, jejunum and ileum were grown for 2 weeks and then incubated with or without WNT3A for 7 days. (A) qRT-PCR for Gata4, Sis (sucrase isomaltase) and Slc10a2 (ASBT). Data from two independent experiments were normalized to Gapdh housekeeping mRNA levels and are represented as fold induction relative to cultures without WNT ± SEM; ***p < 0.005. (B) Differential interference contrast (DIC) pictures of the organoid cultures in presence or absence of WNT for 7 days after splitting. Original magnification 10x.|
|stem1655-sup-0004-suppfig2.tif||32941K||Supplementary Figure 2. Differential interference contrast (DIC) pictures from human duodenum organoid cultures. Organoids were generated from human duodenal biopsies and maintained in expansion medium (EM) for 7 weeks. Differentiation was induced by incubation in differentiation medium (DM) for 5 days.|
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