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Additional Supporting Information may be found in the online version of this article.

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STEM_1684_sm_supptable1.pdf15KTable S1, related to Fig 3: Candidate genes relevant to the various processes of hematopoietic reconstitution. List of genes relevant in each stage of hematopoietic reconstitution used in cluster analysis Fig S5.
STEM_1684_sm_supptable2.pdf48KTable S2: Changes in candidate miRNA levels following reconstitution. List of changes in miRNA expression during engraftment of HSC in xenotransplantation with miRNAs being either A. downregulated or turned off versus B. miRNAs becoming detected i.e. turned ON.
STEM_1684_sm_supptable3.pdf17KTable S2. Top 20 ranked targets for each miRNA candidate.
STEM_1684_sm_supplFig1.pdf197KFigure S1, related to Fig 1: Characterization of iPSCs and analysis of pluripotency. A. Reprogramming of fibroblasts to iPSCs induced a transition to hESC-like colony morphology. B. Flow cytometry analysis of hESCs and iPSCs for pluripotency markers including Oct4, Tra 1-81, SSEA3 and SSEA4. C. Intratesticular delivery of iPSCs in immuno-deficient mouse hosts generated teratomas, which display all three germ layers. D. Hematopoietic development of two iPSC lines derived from human fibroblasts relative to H9 hESC. Scale bar, 500μm. E. Quantitative PCR showing the levels of total Lin28 of hiPSC relative H9 hESC confirming silencing of this reprogramming factor.
STEM_1684_sm_supplFig2.pdf71KFigure S2, related to Fig 2: Engraftment of human cells in xenotransplantation models. A. Adult HSCs readily engrafted NOD-SCID recipients and can be detected in the injected femur and the contralateral bones, unlike hESC or iPSCs. B. Whole gel PCR showing human specific Chromosome 17 expression in the injected femur (F), contralateral bones (C) or spleen (S) of NSG mice transplanted with HSC, hESC-derived or hiPSC-derived cells.
STEM_1684_sm_supplFig3.pdf160KFigure S3, related to Fig 2: Analysis of hiPSC-graft cells. A. iPSC-derived graft cells were also devoid of several lineage specific markers including CD33 (myeloid), CD3 (T-cell) and CD19 (B-cell). For comparison, cells extracted from mice injected with HSCs are also presented. B. Live-dead discrimination plots of hiPSC-graft cells showing the presence of HLA-A/B/C.
STEM_1684_sm_supplFig4.pdf44KFigure S4, related to Fig 3: Image of colony forming units (CFU). CFUs generated from A. hiPSC-derived graft cells or B. HSC graft cells recovered from xenotransplantation recipients.
STEM_1684_sm_supplFig5.pdf66KFigure S5, related to Fig 3: Cluster analysis of various processes involved in hematopoietic reconstitution.
STEM_1684_sm_supplFig6.pdf50KFigure S6. related to Fig 3: miRNA heat map of hESC-derivatives. miRNA heat map of hESC-derivatives and their comparison to HSC and engrafted HSCs.

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