sdf1 Expression Reveals a Source of Perivascular-Derived Mesenchymal Stem Cells in Zebrafish

Authors

  • Troy C. Lund,

    Corresponding author
    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
    • Correspondence: Troy Lund, Ph.D., M.S., M.D., F.A.A.P., University of Minnesota, Pediatric Blood and Marrow Transplant Program, Stem Cell Institute, Global Pediatrics, MMC 366, 420 Delaware St SE, Minneapolis, Minnesota 55455, USA. Telephone: 612-625-4185; Fax: 612-624-3913; e-mail: lundx072@umn.edu

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  • Xiaobai Patrinostro,

    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
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  • Ashley C. Kramer,

    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
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  • Paul Stadem,

    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
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  • Lee Ann Higgins,

    1. Department of Biochemistry, University of Minnesota, Minneapolis, Minnesota, USA
    2. Department of Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA
    3. Department of Biophysics, University of Minnesota, Minneapolis, Minnesota, USA
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  • Todd W. Markowski,

    1. Department of Biochemistry, University of Minnesota, Minneapolis, Minnesota, USA
    2. Department of Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA
    3. Department of Biophysics, University of Minnesota, Minneapolis, Minnesota, USA
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  • Matt S. Wroblewski,

    1. Department of Biochemistry, University of Minnesota, Minneapolis, Minnesota, USA
    2. Department of Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA
    3. Department of Biophysics, University of Minnesota, Minneapolis, Minnesota, USA
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  • Diane S. Lidke,

    1. Department of Pathology and Cancer Research Center and Treatment Center, University of New Mexico, Albuquerque, New Mexico, USA
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  • Jakub Tolar,

    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
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  • Bruce R. Blazar

    1. Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA
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Abstract

There is accumulating evidence that mesenchymal stem cells (MSCs) have their origin as perivascular cells (PVCs) in vivo, but precisely identifying them has been a challenge, as they have no single definitive marker and are rare. We have developed a fluorescent transgenic vertebrate model in which PVC can be visualized in vivo based upon sdf1 expression in the zebrafish. Prospective isolation and culture of sdf1DsRed PVC demonstrated properties consistent with MSC including prototypical cell surface marker expression; mesodermal differentiation into adipogenic, osteogenic, and chondrogenic lineages; and the ability to support hematopoietic cells. Global proteomic studies performed by two-dimensional liquid chromatography and tandem mass spectrometry revealed a high degree of similarity to human MSC (hMSC) and discovery of novel markers (CD99, CD151, and MYOF) that were previously unknown to be expressed by hMSC. Dynamic in vivo imaging during fin regeneration showed that PVC may arise from undifferentiated mesenchyme providing evidence of a PVC-MSC relationship. This is the first model, established in zebrafish, in which MSC can be visualized in vivo and will allow us to better understand their function in a native environment. Stem Cells 2014;32:2767–2779

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