• Open Access

3D Timelapse Analysis of Muscle Satellite Cell Motility§

Authors

  • Ashley L. Siegel,

    1. Division of Biology,University of Missouri, Columbia, Missouri, USA
    2. Christopher H. Bond Life Sciences Center,University of Missouri, Columbia, Missouri, USA
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  • Kevin Atchison,

    1. Division of Biology,University of Missouri, Columbia, Missouri, USA
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  • Kevin E. Fisher,

    1. Department of Medical Pharmacology and Physiology, and University of Missouri, Columbia, Missouri, USA
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  • George E. Davis,

    1. Department of Medical Pharmacology and Physiology, and University of Missouri, Columbia, Missouri, USA
    2. Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, Missouri, USA
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  • D.D.W. Cornelison

    Corresponding author
    1. Division of Biology,University of Missouri, Columbia, Missouri, USA
    2. Christopher H. Bond Life Sciences Center,University of Missouri, Columbia, Missouri, USA
    • 340F LSC, 1201 E Rollins Rd, Columbia, MO 65211, USA

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    • Telephone: (573) 882-9690, FAX: (573) 884-0802


  • Author contributions: A.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; K.A.: collection and/or assembly of data; K.E.F.: conception and design, manuscript writing; G.E.D.: conception, data analysis and interpretation, manuscript writing; D.D.W.C.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLS EXPRESS July 16, 2009; available online without subscription through the open access option.

Abstract

Skeletal muscle repair and regeneration requires the activity of satellite cells, a population of myogenic stem cells scattered throughout the tissue and activated to proliferate and differentiate in response to myotrauma or disease. While it seems likely that satellite cells would need to navigate local muscle tissue to reach damaged areas, relatively little data on such motility exist, and most studies have been with immortalized cell lines. We find that primary satellite cells are significantly more motile than myoblast cell lines, and that adhesion to laminin promotes primary cell motility more than fourfold over other substrates. Using timelapse videomicroscopy to assess satellite cell motility on single living myofibers, we have identified a requirement for the laminin-binding integrin α7β1 in satellite cell motility, as well as a role for hepatocyte growth factor in promoting directional persistence. The extensive migratory behavior of satellite cells resident on muscle fibers suggests caution when determining, based on fixed specimens, whether adjacent cells are daughters from the same mother cell. We also observed more persistent long-term contact between individual satellite cells than has been previously supposed, potential cell-cell attractive and repulsive interactions, and migration between host myofibers. Based on such activity, we assayed for expression of “pathfinding” cues, and found that satellite cells express multiple guidance ligands and receptors. Together, these data suggest that satellite cell migration in vivo may be more extensive than currently thought, and could be regulated by combinations of signals, including adhesive haptotaxis, soluble factors, and guidance cues. STEM CELLS 2009;27:2527–2538

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