Concise Review: Tissue-Specific Microvascular Endothelial Cells Derived From Human Pluripotent Stem Cells

Authors

  • Hannah K. Wilson,

    1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA
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  • Scott G. Canfield,

    1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA
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  • Eric V. Shusta,

    Corresponding author
    1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA
    • Correspondence: Sean P. Palecek, Ph.D., Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA. Telephone: 608-262-8931; Fax: 608-262-5434; e-mail: palecek@engr.wisc.edu; or Eric V. Shusta, Ph.D., Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA. Telephone: 608-262-1092; Fax: 608-262-5434; e-mail: shusta@engr.wisc.edu

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  • Sean P. Palecek

    Corresponding author
    1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA
    • Correspondence: Sean P. Palecek, Ph.D., Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA. Telephone: 608-262-8931; Fax: 608-262-5434; e-mail: palecek@engr.wisc.edu; or Eric V. Shusta, Ph.D., Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA. Telephone: 608-262-1092; Fax: 608-262-5434; e-mail: shusta@engr.wisc.edu

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Abstract

Accumulating evidence suggests that endothelial cells (ECs) display significant heterogeneity across tissue types, playing an important role in tissue regeneration and homeostasis. Recent work demonstrating the derivation of tissue-specific microvascular endothelial cells (TS-MVECs) from human pluripotent stem cells (hPSCs) has ignited the potential to generate tissue-specific models which may be applied to regenerative medicine and in vitro modeling applications. Here, we review techniques by which hPSC-derived TS-MVECs have been made to date and discuss how current hPSC-EC differentiation protocols may be directed toward tissue-specific fates. We begin by discussing the nature of EC tissue specificity in vivo and review general hPSC-EC differentiation protocols generated over the last decade. Finally, we describe how specificity can be integrated into hPSC-EC protocols to generate hPSC-derived TS-MVECs in vitro, including EC and parenchymal cell coculture, directed differentiation, and direct reprogramming strategies. Stem Cells 2014;32:3037–3045

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