SEARCH

SEARCH BY CITATION

Keywords:

  • Neurodegeneration;
  • Stem cells;
  • Extracellular matrix;
  • Proteoglycans;
  • Preclinical trials

ABSTRACT

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder resulting in a lethal outcome. We studied changes in ventral horn perineuronal nets (PNN) of superoxide dismutase 1 (SOD1) rats during the normal disease course and after the intrathecal application (5×105 cells) of human bone marrow mesenchymal stromal cells (MSCs) post symptom manifestation. We found that MSCs ameliorated disease progression, significantly improved motor activity and prolonged survival. For the first time, we report that SOD1 rats have an abnormal disorganized PNN structure around the spinal motoneurons and give different expression profiles of chondroitin sulfate proteoglycans (CSPGs), such as versican, aggrecan and phosphacan, but not link protein-1. Additionally, SOD1 rats had different profiles for CSPG gene expression (Versican, Hapln1, Neurocan and Tenascin-R), whereas Aggrecan and Brevican profiles remained unchanged. The application of MSCs preserved PNN structure, accompanied by better survival of motorneurons. We measured the concentration of cytokines (IL-1α, MCP-1, TNF-α, GM-CSF, IL-4 and IFN-γ) in the rats’ cerebrospinal fluid and found significantly higher concentrations of IL-1α and MCP-1. Our results show that PNN and cytokine homeostasis are altered in the SOD1 rat model of ALS. These changes could potentially serve as biological markers for the diagnosis, assessment of treatment efficacy and prognosis of ALS. We also show that the administration of human MSCs is a safe procedure that delays the loss of motor function and increases the overall survival of symptomatic ALS animals, by remodelling the recipients’ pattern of gene expression and having neuroprotective and immunomodulatory effects. Stem Cells 2014