Tissue-Specific Stem Cells

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Rostro-Caudal Gradual Loss of Cellular Diversity Within the Periventricular Regions of the Ventricular System†‡

  1. Andreas Hermann1,2,
  2. Christian Suess1,
  3. Mareike Fauser1,
  4. Sylvia Kanzler1,
  5. Martin Witt1,3,4,
  6. Klaus Fabel2,5,
  7. Johannes Schwarz6,7,
  8. Günter U. Höglinger8,
  9. Alexander Storch1,2,§,*

Article first published online: 29 JAN 2009

DOI: 10.1002/stem.21

Issue

STEM CELLS

STEM CELLS

Volume 27, Issue 4, pages 928–941, April 2009

Additional Information

How to Cite

Hermann, A., Suess, C., Fauser, M., Kanzler, S., Witt, M., Fabel, K., Schwarz, J., Höglinger, G. U. and Storch, A. (2009), Rostro-Caudal Gradual Loss of Cellular Diversity Within the Periventricular Regions of the Ventricular System. STEM CELLS, 27: 928–941. doi: 10.1002/stem.21

Author Information

  1. 1

    Department of Neurology, Dresden University of Technology, Dresden, Germany

  2. 2

    Center for Regenerative Therapies Dresden, Dresden, Germany

  3. 3

    Department of Anatomy and Cell Biology, Dresden University of Technology, Dresden, Germany

  4. 4

    Department of Otorhinolaryngology, Dresden University of Technology, Dresden, Germany

  5. 5

    Department of Psychiatry, Dresden University of Technology, Dresden, Germany

  6. 6

    Department of Neurology, University of Leipzig, Leipzig, Germany

  7. 7

    Division of Biology, California Institute of Technology, Pasadena, California, USA

  8. 8

    Department of Experimental Neurology, Philipps-University, Marburg, Germany

  1. §

    Telephone: ++49-51-458-2532; Fax: ++49-351-458-4352

*Department of Neurology and Center for Regenerative Therapies Dresden (CRTD), Dresden University of Technology, Fetscherstraße 74, 01307 Dresden, Germany

  1. †

    First published online in STEM CELLSExpress January 29, 2009.

  2. ‡

    Author contributions: A.H.: conception and design, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing; C.S.: design, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing; M.F.: collection and/or assembly of data, data analysis and interpretation; S.K.: collection and/or assembly of data, data analysis and interpretation; M.W.: electron microscopy, data analysis and interpretation, manuscript writing; K.F.: provision of study material, manuscript writing; J.S.: provision of study material, data analysis and interpretation, manuscript writing; G.U.H.: provision of study material, data analysis and interpretation, manuscript writing. A.S.: conception and design, principal investigator and grant support, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing.

Publication History

  1. Issue published online: 6 APR 2009
  2. Article first published online: 29 JAN 2009
  3. Accepted manuscript online: 29 JAN 2009 12:00AM EST
  4. Manuscript Accepted: 19 JAN 2009
  5. Manuscript Received: 14 OCT 2008

Funded by

  • Deutsche Parkinson-Gesellschaft (DPG)
  • Glaxo-SmithKline fellowship
  • Medical Faculty Research Program MeDDrive
  • DFG-Research Center for Regenerative Therapies Dresden (CRTD)
Corrected by:

Erratum: Erratum to: Rostro-Caudal Gradual Loss of Cellular Diversity Within the Periventricular Regions of the Ventricular System

Vol. 28, Issue 4, 843, Article first published online: 14 APR 2010

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Keywords:

  • Adult neural stem cells;
  • Midbrain neurogenesis;
  • Parkinson's disease;
  • Ventricular zone;
  • Neuroepithelial cells

Abstract

Neurogenesis occurs constitutively within the periventricular region (PVR) of the lateral ventricles (LV) of the adult mammalian brain. The occurrence of adult neurogenesis within the PVR outside the neurogenic niche of the LV remains controversial, but neural stem cells can be isolated from PVR of the whole ventricular system. The histological basis of this phenomenon including the regional differences of cellular phenotypes within the PVRs is still enigmatic. The occurrence of neurogenesis or manipulable progenitor cells in caudal parts of the adult brain is however one prerequisite for orthotopic regenerative approaches in Parkinson's disease (PD) and other disorders of the midbrain/brainstem. Using quantitative immunohistochemical techniques and electron microscopy, we found a rostro-caudal gradual loss of cellular diversity within the PVR throughout the whole ventricular axis with loss of transit amplifying epidermal growth factor-receptor+ type C cells in all parts caudal to the LV, a gradual reduction from rostral to caudal of both stem cells (type B cells or astrocytes) without signs of proliferation outside the PVR of the LV as well as neuroblasts-like cells (polysialylated neural cell adhesion molecule [PSA-NCAM]+, but doublecortin negative cells) with a different morphology compared with neuroblasts of the PVR of the LV. Electron microscopy confirmed these immunohistochemical data. The proportion of Nestin+/CD24+ cells and Nestin+/S100β+ ependymal cells were consecutively increased in the PVR from rostral to caudal, and ultrastructural analysis showed a region-specific morphology with darker cytoplasm with occasional large lipid droplets as well as indented nuclei within the caudal PVRs. The strong correlation of neuroblast-like cells with the number of neurosphere-forming cells suggests that a quiescent subtype of PSA-NCAM+ cells might be a source of neurosphere-forming cells. We did not find any evidence for neurogenesis or the occurrence of neuroprogenitors within the substantia nigra or other parts of the midbrain/brainstem outside the PVR. Our data provide the histological framework for future studies on orthotopic regenerative approaches in PD by recruiting endogenous predopaminergic progenitors from the midbrain PVR. STEM CELLS 2009;27:928–941

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