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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 8 OCT 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 12, pages 2962–2968, December 2009
How to Cite
Do, J. T., Joo, J. Y., Han, D. W., Araúzo-Bravo, M. J., Kim, M. J., Greber, B., Zaehres, H., Sobek-Klocke, I., Chung, H. M. and Schöler, H. R. (2009), Generation of Parthenogenetic Induced Pluripotent Stem Cells from Parthenogenetic Neural Stem Cells. STEM CELLS, 27: 2962–2968. doi: 10.1002/stem.233
Author contributions: J.T.D.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; J.Y.J.: conception and design, collection and/or assembly of data; D.W.H.: collection and/or assembly of data, data analysis and interpretation; M.J.A.-B.: data analysis and interpretation; M.J.K.: collection and/or assembly of data; B.G.: data analysis and interpretation; H.Z. and I.S.-K.: provision of study material or patients; H.M.C.: provision of study material or patients, financial support; H.R.S.: conception and design, financial support, manuscript writing, final approval of manuscript.
First published online in STEM CELLS EXPRESS October 8, 2009.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue published online: 14 DEC 2009
- Article first published online: 8 OCT 2009
- Accepted manuscript online: 8 OCT 2009 12:00AM EST
- Manuscript Accepted: 18 SEP 2009
- Manuscript Received: 13 AUG 2009
- Parthenogenetic maternal iPS cells;
- Genomic imprinting
Somatic cells can achieve a pluripotent cell state in a process called pluripotential reprogramming. Multipotent stem cells can differentiate into cells of only one lineage, but pluripotent stem cells can give rise to cells of all three germ layers of an organism. In this study, we generated induced pluripotent stem (iPS) cells from bimaternal (uniparental) parthenogenetic neural stem cells (pNSCs) by transduction with either four (4F: Oct4, Klf4, Sox2, and c-Myc) or two (2F: Oct4 and Klf4) transcription factors. The resultant maternal iPS cells, which were reprogrammed directly from pNSCs, were capable of generating germ line-competent chimeras. Interestingly, analysis of global gene expression and imprinting status revealed that parthenogenetic iPS cells clustered closer to parthenogenetic ESCs than to female ESCs, with patterns that were clearly distinct from those of pNSCs. STEM CELLS 2009;27:2962–2968