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STEM_233_sm_suppinfofigS1.eps225KSupplemental Figure 1. Pairwise scatter plots comparing the global gene expression patterns of pNSCs with fNSCs. Black lines indicate two-fold differences in gene expression levels between the paired cell types. Genes upregulated in ordinate samples compared with abscissa samples are shown in blue; those downregulated are shown in red. The positions of the pluripotent markers (Pou5f1/Oct4, Sox2, Nanog, Klf4, and Lin28 are shown as green dots. Maternally imprinted genes are represented by magenta circles and paternally imprinted genes by cyan circles. The gene expression levels are in log2 scale. Lower expression levels of maternally imprinted genes were observed in pNSCs.
STEM_233_sm_suppinfofigS2.eps160KSupplemental Figure 2. Phase-contrast image of ESC-like colonies generated from pNSCs and fNSCs. ESC-like colonies were first observed on day 6 post-infection in pNSCs and on day 4 post-infection in fNSCs.
STEM_233_sm_suppinfofigS3.eps106KSupplemental Figure 3. Bisulfite sequencing of the Nanog promoter region in pNSCs, pESCs, and miPS-2F cells. Open and filled circles indicate unmethylated and methylated CpGs, respectively.
STEM_233_sm_suppinfofigS4.eps347KSupplemental Figure 4. Pairwise scatter plots comparing global gene expression patterns of pESCs with fESCs; 4 factor-induced iPS cells line (pNSC-iPS-A2) with piPS-2F cells; and fESCs with piPS-2F cells. Black lines indicate two-fold differences in gene expression levels between the paired cell types. Genes upregulated in ordinate samples compared with abscissa samples are shown in blue; those downregulated are shown in red. The positions of the pluripotent markers (Oct4, Sox2, Nanog, Klf4, and Lin28 are shown as green dots. The gene expression levels are in log2 scale. piPS-2F cells more closely resemble pESCs and a 4 factor–induced piPS cells line (pNSC-iPS-A2) than normal fESCs.
STEM_233_sm_suppinfo.doc8212KSupporting Information

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