Embryonic Stem Cells/Induced Pluripotent Stem Cells

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Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2†‡§

  1. Wenlin Li1,
  2. HongYan Zhou1,
  3. Ramzey Abujarour1,
  4. Saiyong Zhu1,
  5. Jin Young Joo2,
  6. Tongxiang Lin1,
  7. Ergeng Hao3,
  8. Hans R. Schöler2,
  9. Alberto Hayek3,
  10. Sheng Ding1,¶,*

Article first published online: 16 OCT 2009

DOI: 10.1002/stem.240

Issue

STEM CELLS

STEM CELLS

Volume 27, Issue 12, pages 2992–3000, December 2009

Additional Information

How to Cite

Li, W., Zhou, H., Abujarour, R., Zhu, S., Young Joo, J., Lin, T., Hao, E., Schöler, H. R., Hayek, A. and Ding, S. (2009), Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2. STEM CELLS, 27: 2992–3000. doi: 10.1002/stem.240

Author Information

  1. 1

    Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA

  2. 2

    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany

  3. 3

    Whittier Institute, Department of Pediatrics, University of California at San Diego, La Jolla, California, USA

  1. Telephone: (858) 784-7376; Fax: (858) 784-9440

*The Scripps Research Institute, Department of Chemistry, SP30-3157, 10550 North Torrey Pines Road, La Jolla, California 92037, USA

  1. †

    Author contributions: S.D.: conception of the study, manuscript writing, supervision of the study; W.L.: conception of the study, manuscript writing, experimentation; R.A.: manuscript writing, experimentation; H.Z., S.Z. and T.L.: experimentation; E.H.: generation of teratomas; A.H.: supervision of E.H.; J.Y.J.: generation of chimera data; H.R.S.: supervision of J.Y.J.

  2. ‡

    First published online in STEM CELLS EXPRESS October 16, 2009.

  3. §

    Disclosure of potential conflicts of interest is found at the end of this article.

Publication History

  1. Issue published online: 14 DEC 2009
  2. Article first published online: 16 OCT 2009
  3. Accepted manuscript online: 16 OCT 2009 12:00AM EST
  4. Manuscript Accepted: 5 OCT 2009
  5. Manuscript Received: 11 MAY 2009

Funded by

  • Fate Therapeutics and CIRM
  • Larry L Hillblom Foundation

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Keywords:

  • Induced pluripotency;
  • iPS;
  • Pluripotent stem cells;
  • Cell culture

Abstract

Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named tranylcypromine), an inhibitor of lysine-specific demethylase 1, CHIR99021 can cause the reprogramming of human primary keratinocyte transduced with the two factors, Oct4 and Klf4. To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. Our studies suggest that the GSK-3 inhibitor might have a general application to replace transcription factors in both mouse and human reprogramming. STEM CELLS 2009;27:2992–3000

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