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Tissue-Specific Stem Cells
Version of Record online: 26 OCT 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 12, pages 3053–3062, December 2009
How to Cite
Balasubramanian, S., Babai, N., Chaudhuri, A., Qiu, F., Bhattacharya, S., Dave, B. J., Parameswaran, S., Carson, S. D., Thoreson, W. B., Sharp, J. G., Rao, M. and Ahmad, I. (2009), Non Cell-Autonomous Reprogramming of Adult Ocular Progenitors: Generation of Pluripotent Stem Cells without Exogenous Transcription Factors. STEM CELLS, 27: 3053–3062. doi: 10.1002/stem.242
Author contributions: S. Balasubramanian: collection and assembly of data; data analysis and interpretation; manuscript writing; N.B., A.C., S. Bhattacharya, and S.P.: collection and assembly of data; F.Q., W.B.T., and M.R.: data analysis and interpretation; B.D. and S.C.: collection and assembly of data; data analysis and interpretation; J.G.S.: provision of study materials; data analysis and interpretation; I.A.: conception and design; data analysis and interpretation; manuscript writing.
First published online in STEM CELLS EXPRESS October 26, 2009.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue online: 14 DEC 2009
- Version of Record online: 26 OCT 2009
- Accepted manuscript online: 26 OCT 2009 12:00AM EST
- Manuscript Accepted: 12 OCT 2009
- Manuscript Received: 9 SEP 2009
- Lincy Foundation, Nebraska Department of Human and Health Services
- Pearson Foundation
- Nebraska Tobacco Fund for Biomedical Research
- Induced pluripotency;
- Pluripotent stem cells;
- Progenitor cells;
- Hepatocyte differentiation;
- Neural induction
Direct reprogramming of differentiated cells to induced pluripotent stem (iPS) cells by ectopic expression of defined transcription factors (TFs) represents a significant breakthrough towards the use of stem cells in regenerative medicine (Takahashi and Yamanaka Cell 2006;126:663–676). However, the virus-mediated expression of exogenous transcription factors could be potentially harmful and, therefore, represents a barrier to the clinical use of iPS cells. Several approaches, ranging from plasmid-mediated TF expression to introduction of recombinant TFs (Yamanaka Cell 2009;137:13–17; Zhou, Wu, Joo et al. Cell Stem Cell 2009;4:381–384), have been reported to address the risk associated with viral integration. We describe an alternative strategy of reprogramming somatic progenitors entirely through the recruitment of endogenous genes without the introduction of genetic materials or exogenous factors. To this end, we reprogrammed accessible and renewable progenitors from the limbal epithelium of adult rat eye by microenvironment-based induction of endogenous iPS cell genes. Non cell-autonomous reprogramming generates cells that are pluripotent and capable of differentiating into functional neurons, cardiomyocytes, and hepatocytes, which may facilitate autologous cell therapy to treat degenerative diseases. STEM CELLS 2009;27:3053–3060