SEARCH

SEARCH BY CITATION

Additional supporting information available online.

FilenameFormatSizeDescription
STEM_242_sm_suppinfofigure1.tif21814KFigure 1. Limbal specificity of progenitors: Sections of adult rat eye through limbal epithelium reveal that cells in the basal region express immunoreactivities corresponding to limbal progenitors markers, ΔP63α (A-C). Cells in the neurospheres prior to iPSC induction expressed immunoreactivities corresponding to ΔP63α (D-F). RT-PCR analysis carried out on these cells revealed that they express transcripts corresponding to limbal progenitor markers, ΔP63α, α-ENOLASE, CYTOKERTIN 15, and MESOTHELIN and not neural crest markers, SLUG, BARX1, and DGRC6 (G). Lane M=marker; lane 1= limbal epithelium cells; 2=limbal neurospheres; 3= neural crest cell line.
STEM_242_sm_suppinfofigure2.tif6715KFigure 2: LE progenitors derived from GFP-mice when cultured in the presence CM derived from mouse ES cells (D3 line) generated colonies, which consist exclusively of GFP+ cells
STEM_242_sm_suppinfofigure3.tif6628KFigure 3. Neuronal differentiation of rat limbal iPSCs. Rat limbal iPSCs were subjected to neuronal differentiation protocol established for mouse ES cells (A). Temporal PCR analysis of stage- and cell type-specific gene expression in limbal iPSCs undergoing neuronal differentiation (B).
STEM_242_sm_suppinfofigure4.tif4513KFigure 4. Cardiomyocyte differentiation of rat limbal iPSCs. Rat limbal iPSCs were subjected to cardiomyocyte differentiation protocol established for mouse ES cells (A). Temporal PCR analysis of stage- and cell type-specific gene expression in limbal iPSCs undergoing cardiomyocyte differentiation (B).
STEM_242_sm_suppinfofigure5.tif8569KFigure 5. Hepatocyte differentiation of rat limbal iPSCs. Rat limbal iPSCs were subjected to hepatocyte differentiation protocol established for mouse ES cells (A). Temporal PCR analysis of stage- and cell type-specific gene expression in limbal iPSCs undergoing hepatocyte differentiation (B).
STEM_242_sm_suppinfofigure6a.mp43260KFigure 6. Beating colonies of cardiomyocytes. Rat limbal iPSCs when subjected to cardiomyocytes differentiation protocol generated colonies 4DIV with spontaneous beating properties (A).
STEM_242_sm_suppinfofigure6b.mp43177KCells from beating colonies displayed contractile properties upon culture (B).
STEM_242_sm_suppinfotables.doc90KTable 1: Gene-specific primers for RT and Q-PCR. Table 2: Primers for bisulfite genomic sequencing.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.