Tissue-Specific Stem Cells

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Brief Report: Interferon-γ Induces Expansion of Lin−Sca-1+C-Kit+ Cells†‡§

  1. Xin Zhao1,
  2. Guangwen Ren2,
  3. Li Liang1,
  4. Phillip Z. Ai1,
  5. Betty Zheng1,
  6. Jay A. Tischfield1,
  7. Yufang Shi2,3,¶,*,
  8. Changshun Shao1,4,‖,*

Article first published online: 4 NOV 2009

DOI: 10.1002/stem.252

Issue

STEM CELLS

STEM CELLS

Volume 28, Issue 1, pages 122–126, January 2010

Additional Information

How to Cite

Zhao, X., Ren, G., Liang, L., Ai, P. Z., Zheng, B., Tischfield, J. A., Shi, Y. and Shao, C. (2010), Brief Report: Interferon-γ Induces Expansion of Lin−Sca-1+C-Kit+ Cells. STEM CELLS, 28: 122–126. doi: 10.1002/stem.252

Author Information

  1. 1

    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA

  2. 2

    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey, USA

  3. 3

    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiao Tong University School of Medicine, Shanghai, China

  4. 4

    Key Laboratory of Experimental Teratology, MOE and Institute of Molecular Medicine and Genetics, Shandong University, Jinan, Shandong China

  1. Telephone: (732) 235-4501; Fax: (732) 235-4505

  2. ‖

    Telephone: 732-445-5406; Fax: 732-445-1147

*Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 661 Hoes Lane, Piscataway, NJ 08854, USA

  1. †

    Author contributions: X.Z., G.R.: Conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; L.L.: Collection and assembly of data, and data analysis and interpretation; P.Z.A., B.Z: Collection and assembly of data; J.A.T.: Conception and design, and financial support; Y.S. and C.S.: Conception and design, manuscript writing, final approval of manuscript, and financial support. X.Z. and G.R. contributed equally to this work.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLS EXPRESS November 4, 2009.

Publication History

  1. Issue published online: 12 JAN 2010
  2. Article first published online: 4 NOV 2009
  3. Accepted manuscript online: 4 NOV 2009 12:00AM EST
  4. Manuscript Accepted: 27 OCT 2009
  5. Manuscript Received: 24 AUG 2009

Funded by

  • New Jersey Commission on Science and Technology

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Keywords:

  • Hematopoietic progenitor cells;
  • Lineage−;
  • Lineage−Sca-1+C-kit+ cells;
  • Interferon-γ

Abstract

The balance between Th1 and Th2 cells is critical for homeostasis of the immune system. Th1 cells can also regulate hematopoietic progenitor cell homeostasis by production of oncostatin M. Here we show that Th1 cell products, but not those of Th2 cells, caused a rapid expansion of lineage−Sca-1+C-kit+ (LSK) cells in vivo and in vitro. Among Th1 cytokines, interferon-γ (IFNγ) was found to play a major role in this expansion by activating the expression of Sca-1 in lineage−Sca-1−C-kit+ cells. This process was dependent on IFNγR1 signaling and the STAT1 pathway. Furthermore, those IFNγ-induced LSK cells had a higher proliferation potential than control LSK cells. In addition, while the overall production of colony-forming units in bone marrow was decreased after IFNγ treatment, the sorted LSK cells could give rise to a higher yield of colony-forming units. Finally, the IFNγ-induced hematopoiesis was biased toward the differentiation of myeloid lineages. Therefore, our findings demonstrated a novel role of IFNγ in activating hematopoietic progenitor cells and provide a new insight into the clinical application of interferon. STEM CELLS 2010;28:122–126

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