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Tissue-Specific Stem Cells
Article first published online: 18 NOV 2009
Copyright © 2009 AlphaMed Press
Volume 28, Issue 1, pages 140–151, January 2010
How to Cite
Sordi, V., Melzi, R., Mercalli, A., Formicola, R., Doglioni, C., Tiboni, F., Ferrari, G., Nano, R., Chwalek, K., Lammert, E., Bonifacio, E. and Piemonti, L. (2010), Mesenchymal Cells Appearing in Pancreatic Tissue Culture Are Bone Marrow-Derived Stem Cells With the Capacity to Improve Transplanted Islet Function. STEM CELLS, 28: 140–151. doi: 10.1002/stem.259
Author contributions: V.S.: Conception and design, collection and assembly of data, manuscript writing; R.M., A.M., R.F., C.D., E.L.: Collection and assembly of data; G.F.: Provision of study material, collection and assembly of data; B.A., R.N.: Provision of study material; K.C., E.B.: Conception and design, manuscript writing, final approval of manuscript; L.P.: Conception and design, data analysis and interpretation, manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS November 18, 2009.
- Issue published online: 12 JAN 2010
- Article first published online: 18 NOV 2009
- Accepted manuscript online: 18 NOV 2009 12:00AM EST
- Manuscript Accepted: 6 NOV 2009
- Manuscript Received: 3 JUN 2009
- EU (DIAPREPP Project, HEALTH-F2-2008-202013)
- Telethon Italy and the Juvenile Diabetes Research Foundation. Grant Number: JT01Y01
- Italian Telethon Foundation (TIGET grant)
- Award from Fondazione Cariplo. Grant Number: 2007-5165
- Fondazione Siemens Italia (Milan, Italy)
Vol. 28, Issue 2, 386, Article first published online: 16 FEB 2010
- Mesenchymal cells;
- Pancreatic tissue;
- Islet function
Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts to differentiate human β cells from pancreatic precursors. A thorough characterization of these mesenchymal cells has not yet been completed, and there are no conclusive data about their origin.
We demonstrated that the human mesenchymal cells outgrowing from cultured human pancreatic endocrine or exocrine tissue are pancreatic mesenchymal stem cells (pMSC) that propagate from contaminating pMSC. The origin of pMSC is partly extrapancreatic both in humans and mice, and by using green fluorescent protein (GFP+) bone marrow transplantation in the mouse model, we were able to demonstrate that these cells derive from the CD45+ component of bone marrow. The pMSC express negligible levels of islet-specific genes both in basal conditions and after serum deprivation or exogenous growth factor exposure, and might not represent optimal candidates for generation of physiologically competent β-cells. On the other hand, when cotransplanted with a minimal pancreatic islet mass, pMSC facilitate the restoration of normoglycemia and the neovascularization of the graft. These results suggest that pMSCs could exert an indirect role of “helper” cells in tissue repair processes. STEM CELLS 2010;28:140–151