Embryonic Stem Cells/Induced Pluripotent Stem Cells

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Characterization of Gastrulation-Stage Progenitor Cells and Their Inhibitory Crosstalk in Human Embryoid Bodies†‡§

  1. Oded Kopper,
  2. Odil Giladi,
  3. Tamar Golan-Lev,
  4. Nissim Benvenisty¶,*

Article first published online: 17 NOV 2009

DOI: 10.1002/stem.260

Issue

STEM CELLS

STEM CELLS

Volume 28, Issue 1, pages 75–83, January 2010

Additional Information

How to Cite

Kopper, O., Giladi, O., Golan-Lev, T. and Benvenisty, N. (2010), Characterization of Gastrulation-Stage Progenitor Cells and Their Inhibitory Crosstalk in Human Embryoid Bodies. STEM CELLS, 28: 75–83. doi: 10.1002/stem.260

Author Information

  1. Stem Cell Unit, Department of Genetics, The Institute of Life Sciences, The Hebrew University, Edmund J. Safra Campus, Givat Ram, Jerusalem, Israel

  1. Telephone: +972-2-6586774; Fax: +972-2-6584972

*Stem Cell Unit, Department of Genetics, The Institute of Life Sciences, The Hebrew University, Edmund J. Safra Campus, Givat Ram, Jerusalem, 91904, Israel

  1. †

    Author contributions: O.K.: Conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing; O.G.: Collection and assembly of data; T.G.-L.: Collection and assembly of data; N.B.: Conception and design, financial support, data analysis and interpretation, manuscript writing, final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLS EXPRESS November 17, 2009.

Publication History

  1. Issue published online: 12 JAN 2010
  2. Article first published online: 17 NOV 2009
  3. Accepted manuscript online: 17 NOV 2009 12:00AM EST
  4. Manuscript Accepted: 3 NOV 2009
  5. Manuscript Received: 23 AUG 2009

Funded by

  • Israeli Ministry of Trade and Industry. Grant Number: 37675
  • European Community (ESTOOLS). Grant Number: 018739
  • Legacy Heritage Fund of New York

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Keywords:

  • Embryogenesis;
  • Human embryoid bodies;
  • Human embryonic stem cells;
  • Progenitor cells

Abstract

Human embryoid bodies (HEBs) are cell aggregates that are produced during the course of embryonic stem cell differentiation in suspension. Mature HEBs have been shown to contain derivatives of the three embryonic germ layers. In this study, using a combination of laser capture microscopy followed by DNA microarray analysis and cell sorting, we demonstrate that early HEBs are composed of three major cell populations. These cell populations can be defined by the expression of specific cell markers, namely: (i) OCT4+, REX1−; (ii) NCAD+, OCT4−; and (iii) EPOR+, OCT4−. By analyzing gene expression in embryonic tissues, these cell populations could respectively be assigned to the embryonic ectoderm, mesendoderm, and extraembryonic endoderm lineages. We show that the extraembryonic endoderm, which selectively expresses platelet-derived growth factor B (PDGF-B), negatively affects the mesendoderm lineage, which selectively expresses the receptor PDGFRA. Our analysis suggests that early HEBs are spatially patterned and that cell differentiation is governed by interactions between the different cell types. STEM CELLS 2010;28:75–83

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