Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Authors

  • Nuria Gago,

    1. Rare Disease Program, Skin Regeneration Laboratory, Fundación Inbiomed, Paseo Mikeletegi 61, San Sebastián, Spain
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  • Virginia Pérez-López,

    1. Rare Disease Program, Skin Regeneration Laboratory, Fundación Inbiomed, Paseo Mikeletegi 61, San Sebastián, Spain
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  • Juan Pablo Sanz-Jaka,

    1. Departments of Urology and Paediatric Surgery, Hospital Donostia, Avenida Dr. Beguiristain, San Sebastián, Spain
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  • Pedro Cormenzana,

    1. Aesthetic Surgery, Policlínica Gipuzkoa, Parque Tecnológico Miramón, San Sebastián, Spain
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  • Iñaki Eizaguirre,

    1. Departments of Urology and Paediatric Surgery, Hospital Donostia, Avenida Dr. Beguiristain, San Sebastián, Spain
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  • Antonio Bernad,

    1. Regenerative Cardiology Department, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fdez. Almagro 3, Madrid, Spain
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  • Ander Izeta

    Corresponding author
    1. Rare Disease Program, Skin Regeneration Laboratory, Fundación Inbiomed, Paseo Mikeletegi 61, San Sebastián, Spain
    2. Regenerative Medicine Program, Instituto Biodonostia, Hospital Donostia, Avenida Dr. Beguiristain, San Sebastián, Spain
    • Fundación Inbiomed, Paseo Mikeletegi 61, 20009 San Sebastián, Spain
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    • Telephone: +34-94-3309064; Fax: +34-94-3308222


  • Author contributions: N.G.: conception and design, collection and/or assembly of data, data analysis and interpretation, final approval of manuscript; V.P.-L.: collection and/or assembly of data, final approval of manuscript; J.P.S.-J.: provision of study material or patients, final approval of manuscript; P.C.: provision of study material or patients, final approval of manuscript; I.E.: provision of study material or patients, final approval of manuscript; A.B.: conception and design, data analysis and interpretation, final approval of manuscript; A.I.: conception and design, financial support, data analysis and interpretation, manuscript writing, final approval of manuscript.

  • First published online in Stem CellsExpress February 5, 2009

Abstract

A major unanswered question in autologous cell therapy is the appropriate timing for cell isolation. Many of the putative target diseases arise with old age and previous evidence, mainly from animal models, suggests that the stem/progenitor cell pool decreases steadily with age. Studies with human cells have been generally hampered to date by poor sample availability. In recent years, several laboratories have reported on the existence, both in rodents and humans, of skin-derived precursor (SKP) cells with the capacity to generate neural and mesodermal progenies. This easily obtainable multipotent cell population has raised expectations for their potential use in cell therapy of neurodegeneration. However, we still lack a clear understanding of the spatiotemporal abundance and phenotype of human SKPs. Here we show an analysis of human SKP abundance and in vitro differentiation potential, by using SKPs isolated from four distinct anatomic sites (abdomen, breast, foreskin, and scalp) from 102 healthy subjects aged 8 months to 85 years. Human SKP abundance and differentiation potential decrease sharply with age, being extremely difficult to isolate, expand, and differentiate when obtained from the elderly. Our data suggest preserving human SKP cell banks early in life would be desirable for use in clinical protocols in the aging population. Stem Cells 2009;27:1164–1172

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