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STEM_277_sm_suppinfoFigS1.tif1410KFigure S1. BM-MSC transplantation had a sustained effect on microglia activation in APP/PS1 mice. Six weeks after the final treatment, microglia activation were assessed by immunofluorescence. Representative immunohistochemical staining showed that expression of Iba-1 positive cells were still increased in the BM-MSC treated APP/PS1 mice (B, D) compare to the PBS injected animals (A, C).
STEM_277_sm_suppinfoFigS2.tif2260KFigure S2. Expression of A?-degrading enzymes and A?-binding receptors were decreased in APP/PS1 mice with age. (A-C) Expression of A?-degrading enzymes in APP/PS1 mice was compared with their age-matched WT littermates at 5, 7, 10, 15 months of age. Expression of IDE (A), NEP (B), and MMP9 (C) was assessed by quantitative real-time PCR analysis. With age, the reduction of these enzymes in RNA levels was statistically significant. Data indicates mean ± SD. (D-F) Expression of A?-binding receptors was compared between APP/PS1 mice and their age-matched WT littermates at 5, 7, 10, 15 months of age. With age, APP/PS1 mice showed significantly reduced expression of MARCO (D) and SRB1 (E) compared with WT littermates. There were no significant differences in expression of CD36 observed between APP/PS1 and WT mice at any age. Data are displayed for APP/PS1 mice as fold changes of their WT littermates. Data indicates mean ± SD.
STEM_277_sm_suppinfoFigS3.tif2054KFigure S3. Transplantation of BM-MSC exert a sustained effect on the alternatively activated microglia. Expression of IL-4 (A), Arg-1 (B), and YM-1 (C) was measured by quantitative real-time PCR in BM-MSC- or PBS-treated APP/PS1 mice (6 weeks after the final treatment). In BM-MSC treated mice, the expression of YM-1 (C) was significantly higher than in the PBS infused animals. In BM-MSC transplanted mice, the mRNA levels of IL-4 (A) and Arg-1 (B) were still higher than in the PBS infused animals, although this did not reach statistical significance. Data indicates mean ± SD. Student's t-test was used for statistical analysis. *P < 0.01 compared with PBS treated APP/PS1 mice.

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