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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 14 DEC 2009
Copyright © 2010 AlphaMed Press
Volume 28, Issue 2, pages 221–228, February 2010
How to Cite
Tsai, S.-Y., Clavel, C., Kim, S., Ang, Y.-S., Grisanti, L., Lee, D.-F., Kelley, K. and Rendl, M. (2010), Oct4 and Klf4 Reprogram Dermal Papilla Cells into Induced Pluripotent Stem Cells. STEM CELLS, 28: 221–228. doi: 10.1002/stem.281
Author contributions: S.Y.T.: conception and design, collection of data, data analysis and interpretation, manuscript writing; C.C., S.K.,Y.S.A., L.G., and D.F.L.: collection of data, data analysis and interpretation; C.C. and S.K. contributed equally to this work; K.K.: collection of data; M.R.: conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS January 28, 2010.
- Issue published online: 16 FEB 2010
- Article first published online: 14 DEC 2009
- Accepted manuscript online: 14 DEC 2009 12:00AM EST
- Manuscript Accepted: 4 DEC 2009
- Manuscript Received: 1 OCT 2009
- Dermatology Foundation Research Career Development Award
- Family Stem Cell Institute
Additional supporting information available online.
|STEM_281_sm_suppinfoFigureS1.tif||2003K||Supplemental online Figure 1. Generation of iPS cells from DP cells with three reprogramming factors. FACS sorted DP cells were 2x infected with retroviruses expressing Oct4, c-Myc, and Klf4 (3TF) and no Sox2. A typical Oct4-GFP positive iPS colony reporting activation of endogenous Oct4 expression is shown (right). The Phase image shows the typical ES/iPS morphology (left).|
|STEM_281_sm_suppinfoFigureS2.tif||2663K||Supplemental online Figure S2. Immunofluorescence staining of pluripotency markers in DP-derived 4TF iPS cells. 4TF iPS cells were stained with antibodies against Nanog, Oct4, Sox2, and SSEA-1 (red). Nuclei were visualized by DAPI (blue).|
|STEM_281_sm_suppinfoFigureS3.tif||5985K||Supplemental online Figure S3. Integration analysis of the four-transgene factors by Southern blotting. Genomic DNA isolated from MEFs, DP cells, 2TF-1, 2TF-10 and 4TF-1 iPS cells were digested with Bgl II, and hybridized with Oct4, Klf4, Sox2 and c-Myc cDNA probes, respectively. Red stars indicate the integration of 1-4 four copies of the exogenous reprogramming genes. The blue triangle denotes the endogenous gene.|
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