Stem Cell Epigenetics, Genomics, and Proteomics

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Conversion of Ancestral Fibroblasts to Induced Pluripotent Stem Cells†‡§

  1. Ryoko Araki1,2,
  2. Yuko Jincho1,
  3. Yuko Hoki1,
  4. Miki Nakamura1,2,
  5. Chihiro Tamura1,
  6. Shunsuke Ando1,
  7. Yasuji Kasama1,
  8. Masumi Abe1,¶,*

Article first published online: 17 DEC 2009

DOI: 10.1002/stem.282

Issue

STEM CELLS

STEM CELLS

Volume 28, Issue 2, pages 213–220, February 2010

Additional Information

How to Cite

Araki, R., Jincho, Y., Hoki, Y., Nakamura, M., Tamura, C., Ando, S., Kasama, Y. and Abe, M. (2010), Conversion of Ancestral Fibroblasts to Induced Pluripotent Stem Cells. STEM CELLS, 28: 213–220. doi: 10.1002/stem.282

Author Information

  1. 1

    Transcriptome Research Group, National Institute of Radiological Sciences, Japan

  2. 2

    PREST, Japan Science and Technology Agency, Japan

  1. Telephone: +81-43-206-3220; Fax: +81-43-251-4593

*Transcriptome Research Group, National Institute of Radiological Sciences, Japan

  1. †

    Author contributions: R.A.: Conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing, financial support; Y.J.: Collection and assembly of data, data analysis and interpretation; Y.H.: Collection and assembly of data, and data analysis; C.T., M.N., and S.A.: Collection and assembly of data; Y.K.: Data analysis; M.A.: Conception and design, financial support, manuscript writing, final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSEXPRESS January 28, 2010.

Publication History

  1. Issue published online: 16 FEB 2010
  2. Article first published online: 17 DEC 2009
  3. Accepted manuscript online: 17 DEC 2009 12:00AM EST
  4. Manuscript Accepted: 2 DEC 2009
  5. Manuscript Received: 8 JUL 2009

Funded by

  • Precursory Research for Embryonic Science and Technology (PRESTO), JST

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Keywords:

  • Induced pluripotent stem cells;
  • Time-lapse imaging;
  • Mice Fibroblasts

Abstract

The emergence of induced pluripotent stem cells (iPSCs) from an ancestral somatic cell is one of the most important processes underlying their generation, but the mechanism has yet to be identified. This is principally because these cells emerge at a low frequency, about 0.1% in the case of fibroblasts, and in a stochastic manner. In our current study, we succeeded in identifying ancestral fibroblasts and the subsequent processes leading to their conversion to iPSCs. The ancestral fibroblasts were found to divide several times in a morphologically symmetric manner, maintaining a fibroblastic shape, and then gradually transform into embryonic stem-like cells. Interestingly, this conversion occurred within 48 hours after gene introduction in most iPSC generations. This is the first report to directly observe a cell lineage conversion of somatic cells to stem cells and provides a critical new insight into the “black box” of iPSCs, that is, the first three days of their generation. STEM CELLS 2010;28:213–220

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