Cancer Stem Cells

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Complex Display of Putative Tumor Stem Cell Markers in the NCI60 Tumor Cell Line Panel†‡§

  1. Christina H. Stuelten1,¶,*,
  2. Susan D. Mertins2,
  3. Johanna I. Busch1,
  4. Meghan Gowens1,
  5. Dominic A. Scudiero3,
  6. Mark W. Burkett3,
  7. Karen M. Hite3,
  8. Mike Alley3,
  9. Melinda Hollingshead4,
  10. Robert H. Shoemaker2,
  11. John E. Niederhuber1

Article first published online: 22 FEB 2010

DOI: 10.1002/stem.324

Issue

STEM CELLS

STEM CELLS

Volume 28, Issue 4, pages 649–660, April 2010

Additional Information

How to Cite

Stuelten, C. H., Mertins, S. D., Busch, J. I., Gowens, M., Scudiero, D. A., Burkett, M. W., Hite, K. M., Alley, M., Hollingshead, M., Shoemaker, R. H. and Niederhuber, J. E. (2010), Complex Display of Putative Tumor Stem Cell Markers in the NCI60 Tumor Cell Line Panel. STEM CELLS, 28: 649–660. doi: 10.1002/stem.324

Author Information

  1. 1

    Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland 20892

  2. 2

    Screening Technologies Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute at Frederick, Frederick, Maryland 21702

  3. 3

    SAIC-Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21702

  4. 4

    Biological Testing Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute at Frederick, Frederick, Maryland 21702

  1. Telephone: (301) 496-0693, Fax: (301) 435-8666

*Cell and Cancer Biology Branch, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Room 1042, Bethesda, MD 20892-4254

  1. †

    Author contributions: C.S.: Conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; S.M.: Conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; J.B. and M.G.: Collection and/or assembly of data, data analysis and interpretation; D.S.: Provision of study material or patients, collection and/or assembly of data; M.B.: Collection and/or assembly of data, manuscript writing; K.H. and M.A.: Collection and/or assembly of data; M.H.: Conception and design of animal studies, collection of data, assembly of data; B.S.: Conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; J.N.: Conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript.

  2. ‡

    First published online in STEM CELLS EXPRESS February 22, 2010.

  3. §

    Disclosure of potential conflicts of interest is found at the end of this article.

Publication History

  1. Issue published online: 14 APR 2010
  2. Article first published online: 22 FEB 2010
  3. Manuscript Accepted: 5 FEB 2010
  4. Manuscript Received: 10 AUG 2009

Funded by

  • National Cancer Institute, National Institutes of Health. Grant Number: HHSN261200800001E
  • Developmental Therapeutics Program in the Division of Cancer Treatment and Diagnosis of the National Cancer Institute

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Keywords:

  • Cancer stem cell;
  • Cell surface marker;
  • Fluorescence-activated cell sorting;
  • Cell culture;
  • NCI60 panel

Abstract

Tumor stem cells or cancer initiating cells (CICs) are single tumor cells that can regenerate a tumor or a metastasis. The identification and isolation of CICs remain challenging, and a variety of putative CIC markers have been described. We hypothesized that cell lines of the NCI60 panel contain CICs and express putative CIC markers. We investigated expression of putative CIC surface markers (CD15, CD24, CD44, CD133, CD166, CD326, PgP) and the activity of aldehyde dehydrogenase in the NCI60 panel singly and in combination by six-color fluorescence-activated cell sorting analysis. All investigated markers were expressed in cell lines of the NCI60 panel. Expression levels of individual markers varied widely across the 60 cell lines, and neither single marker expression nor simple combinations nor co-expression patterns correlated with the colony-formation capacity of cell lines. Rather, marker expression patterns correlated with tumor types in multidimensional analysis. Whereas some expression patterns correlated with tumor entities such as basal breast cancer, other expression patterns occurred across different tumor types and largely related to expression of a more mesenchymal phenotype in individual breast, lung, renal, and melanoma cell lines. Our data for the first time demonstrate that tumor cell lines display CIC markers in a complex pattern that relates to the tumor type. The complexity and tumor type specificity of marker display creates challenges for the application of cell sorting and other approaches to isolation of putative tumor stem cell populations and suggests that therapeutic targeting strategies will need to take this into account. STEM CELLS 2010;28:649–66028:649–660

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