Tissue-Specific Stem Cells
Article first published online: 7 APR 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 5, pages 984–995, May 2010
How to Cite
Marynka-Kalmani, K., Treves, S., Yafee, M., Rachima, H., Gafni, Y., Cohen, M. A. and Pitaru, S. (2010), The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population. STEM CELLS, 28: 984–995. doi: 10.1002/stem.425
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: K.M.-K.: experimental work collection and assembly of data; S.T.: experimental work collection and assembly of data; M.Y.: experimental work collection and assembly of data; H.R.: provision of human oral mucosa biopsies; Y.G.: experimental work collection and assembly of data; M.C.: experimental work collection and assembly of data; S.P.: concept and experimental design and manuscript writing.
First published online in STEM CELLS EXPRESS April 7, 2010.
- Issue published online: 10 MAY 2010
- Article first published online: 7 APR 2010
- Accepted manuscript online: 7 APR 2010 12:00AM EST
- Manuscript Accepted: 26 MAR 2010
- Manuscript Received: 9 AUG 2009
- Israel Science Foundation of the Israeli Academy of Science, Binational United States - Israel Science Foundation, and the Chief Scientist of the Ministry of Health Foundation
- Stem cell;
- Oral mucosa;
- Neural crest;
- Definitive endoderm;
The highly regenerative capacity of the human adult oral mucosa suggests the existence of a robust stem cell (SC) population in its lamina propria (OMLP). The purpose of this study was to characterize the availability, growth, immunophenotype, and potency of this presumable SC population. Cells positive for the embryonic stem cell transcription factors Oct4 and Sox2 and for p75 formed distinct cord-like structure in the OMLP. Regardless of donor age, trillions of cells, termed human oral mucosa stem cells (hOMSC), 95% of which express mesenchymal stromal cell markers, were simply, and reproducibly produced from a biopsy of 3–4 × 2 × 1 mm3. A total of 40–60% of these cells was positive for Oct4, Sox2, and Nanog and 60–80% expressed constitutively neural and neural crest SC markers. hOMSC differentiated in culture into mesodermal (osteoblastic, chondroblastic, and adipocytic), definitive endoderm and ectodermal (neuronal) lineages. Unexpectedly, hOMSC treated with dexamethasone formed tumors consisting of two germ layer-derived tissues when transplanted in severe combined immune deficiency mice. The tumors consisted of tissues produced by neural crest cells during embryogenesis—cartilage, bone, fat, striated muscle, and neural tissue. These results show that the adult OMLP harbors a primitive SC population with a distinct primitive neural-crest like phenotype and identifies the in vivo localization of putative ancestors for this population. This is the first report on ectodermal- and mesodermal-derived mixed tumors formation by a SC population derived from a nonmalignant somatic adult human tissue. STEM CELLS 2010;28:984–995