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Tissue-Specific Stem Cells
Article first published online: 28 APR 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 6, pages 1081–1088, June 2010
How to Cite
Bachelard-Cascales, E., Chapellier, M., Delay, E., Pochon, G., Voeltzel, T., Puisieux, A., Caron de Fromentel, C. and Maguer-Satta, V. (2010), The CD10 Enzyme Is a Key Player to Identify and Regulate Human Mammary Stem Cells. STEM CELLS, 28: 1081–1088. doi: 10.1002/stem.435
Author contributions: E.B.-C.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; M.C.: collection and/or assembly of data, data analysis and interpretation; E.D.: provision of study material or patients; G.P.: collection and/or assembly of data, data analysis and interpretation; T.V.: provision of study material or patients; A.P.: financial support, administrative support; C.C.F.: administrative support, final approval of manuscript; V.M.-S.: conception and design, financial support, administrative support, data analysis and interpretation, manuscript writing, final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS April 28, 2010.
- Issue published online: 8 JUN 2010
- Article first published online: 28 APR 2010
- Accepted manuscript online: 28 APR 2010 12:00AM EST
- Manuscript Accepted: 15 APR 2010
- Manuscript Received: 17 MAR 2010
- INSERM and Ligue Nationale Contre le Cancer (Ain, Saone et Loire)
- Ligue Nationale Contre le Cancer (Rhône) and ARC Associations
- Human breast;
- Stem cell fate;
The major components of the mammary ductal tree are an inner layer of luminal cells, an outer layer of myoepithelial cells, and a basement membrane that separates the ducts from the underlying stroma. Cells in the outer layer express CD10, a zinc-dependent metalloprotease that regulates the growth of the ductal tree during mammary gland development. To define the steps in the human mammary lineage at which CD10 acts, we have developed an in vitro assay for human mammary lineage progression. We show that sorting for CD10 and EpCAM cleanly separates progenitors from differentiated luminal cells and that the CD10-high EpCAM-low population is enriched for early common progenitor and mammosphere-forming cells. We also show that sorting for CD10 enriches sphere-forming cells from other tissue types, suggesting that it may provide a simple tool to identify stem or progenitor populations in tissues for which lineage studies are not currently possible. We demonstrate that the protease activity of CD10 and the adhesion function of β1-integrin are required to prevent differentiation of mammary progenitors. Taken together, our data suggest that integrin-mediated contact with the basement membrane and cleavage of signaling factors by CD10 are key elements in the niche that maintains the progenitor and stem cell pools in the mammary lineage. STEM Cells 2010;28:1081–1088