Transplanted Oligodendrocytes and Motoneuron Progenitors Generated from Human Embryonic Stem Cells Promote Locomotor Recovery After Spinal Cord Transection§

Authors

  • Slaven Erceg,

    Corresponding author
    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
    2. CABIMER (Centro Andaluz de Biología Molecular y Medicina Regenerativa), Avda. Americo Vespucio s/n, Parque Científico y Tecnológico Cartuja, Sevilla, Spain
    • CABIMER (Centro Andaluz de Biología Molecular y Medicina Regenerativa), Avda. Americo Vespucio s/n, Parque Científico y Tecnológico Cartuja, Sevilla, Spain
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    • Telephone: +34-95-446-76-21; Fax: +34-95-446-16-64

  • Mohammad Ronaghi,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Marc Oria,

    1. Liver Unit, Department of Medicine, Hospital Universitari Vall d'Hebron, Universitat Autónoma de Barcelona, Barcelona, Spain
    2. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid, Spain
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  • Mireia García Roselló,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Maria Amparo Pérez Aragó,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Maria Gomez Lopez,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Ivana Radojevic,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Victoria Moreno-Manzano,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Francisco-Javier Rodríguez-Jiménez,

    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
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  • Shom Shanker Bhattacharya,

    1. CABIMER (Centro Andaluz de Biología Molecular y Medicina Regenerativa), Avda. Americo Vespucio s/n, Parque Científico y Tecnológico Cartuja, Sevilla, Spain
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  • Juan Cordoba,

    1. Liver Unit, Department of Medicine, Hospital Universitari Vall d'Hebron, Universitat Autónoma de Barcelona, Barcelona, Spain
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  • Miodrag Stojkovic

    Corresponding author
    1. Cellular Reprogramming Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Valencia, Spain
    2. Spebo Medical, Leskovac, Serbia
    3. Human Genetics, Medical School University of Kragujevac, Serbia
    Current affiliation:
    1. CABIMER (Centro Andaluz de Biología Molecular y Medicina Regenerativa), Avda. Americo Vespucio s/n, Parque Científico y Tecnológico Cartuja, Sevilla, Spain
    • SPEBO Medical, Norvezanska 16, Leskovac, Serbia and Medical Faculty, University of Kragujevac, Kragujevac, Serbia
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    • Telephone: +381-16-21-81-71


  • Author contributions: S.E.: conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript, experiments; M.R.: experiments; M.O.: experiments, data analysis and interpretation; M.G.R.: experiments, surgery; M.A.P.A., M.G.L., I.R., V.M.-M., and F.J.R.-J.: experiments; S.S.B. and J.C.: data analysis and interpretation; M.S.: conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLS EXPRESS July 22, 2010.

Abstract

Human embryonic stem cells (hESC) hold great promise for the treatment of patients with many neurodegenerative diseases particularly those arising from cell loss or neural dysfunction including spinal cord injury. This study evaluates the therapeutic effects of transplanted hESC-derived oligodendrocyte progenitors (OPC) and/or motoneuron progenitors (MP) on axonal remyelination and functional recovery of adult rats after complete spinal cord transection. OPC and/or MP were grafted into the site of injury in the acute phase. Based on Basso-Beattie-Bresnahan scores recovery of locomotor function was significantly enhanced in rats treated with OPC and/or MP when compared with control animals. When transplanted into the spinal cord immediately after complete transection, OPC and MP survived, migrated, and differentiated into mature oligodendrocytes and neurons showing in vivo electrophysiological activity. Taken together, these results indicate that OPC and MP derived from hESC could be a useful therapeutic strategy to repair injured spinal cord. STEM CELLS 2010; 28:1541–1549.

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