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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 27 JUL 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 9, pages 1550–1559, September 2010
How to Cite
Ryul Lee, M., Soo Kim, J. and Kim, K.-S. (2010), miR-124a Is Important for Migratory Cell Fate Transition During Gastrulation of Human Embryonic Stem Cells. STEM CELLS, 28: 1550–1559. doi: 10.1002/stem.490
Author contributions: M.R.L.: conception and design, data analysis and interpretation, manuscript writing; J.S.K.: conception and design, data analysis and interpretation; K.S.K.: conception and design, data analysis and interpretation, administrative support, manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS July 22, 2010.
- Issue published online: 27 JUL 2010
- Article first published online: 27 JUL 2010
- Manuscript Accepted: 19 JUL 2010
- Manuscript Received: 26 FEB 2010
- Stem Cell Research Center. Grant Number: SC-2220
- Medical Research Center. Grant Number: 2009-0091464
- Ministry of Education, Science and Technology, Republic of Korea
- Human embryonic stemcells;
- Cell migration;
Precise control of gene expression is of paramount importance for proper embryonic development. Although a number of microRNAs (miRNAs) has been implicated in fine-tuning mRNA translation during development, their exact roles for gastrulation, particularly in connection with functional targets, have yet to be clarified, with regard to stage-specific cell migration to form three embryonic germ layers. We found that miR-124a is expressed in human embryonic stem cells (hESC), but is gradually downregulated during embryoid body (EB) formation in vitro. We also provide evidence that SLUG and IQGAP1, which modulates rearrangement of the migratory cytoskeleton, are specific targets for miR-124a during EB formation. Furthermore, we show that the beginning of cell migration, a hallmark event in gastrulation, is tightly coupled with downregulation of miR-124a during EB formation and induction of SLUG and IQGAP1. Overexpressed miR-124a in hESC reduced expression of SLUG and IQGAP1 and blocked migratory cell behavior in EB. An expression level of MIXL1, associated with gastulation process, was also inversely correlated with expression of miR-124a. Taken together, our results strongly suggest that miR-124a may play an active role in inhibiting hESCs from differentiation into EB by downregulating expression of SLUG and IQGAP1, thereby maintaining stemness. STEM CELLS 2010; 28:1550–1559.