Additional Supporting Information may be found in the online version of this article.

STEM_498_sm_SuppFig1.tif22791KSupporting Information Figure 1. Differentiation of ES cells into neuronal lineage. Neurons (TuJ1-positive) formed from ES cells after 9 days in N2B27 medium.
STEM_498_sm_SuppFig2.tif325KSupporting Information Figure 2. Relative E-cadherin expression. E-cadherin expression levels in ES cells and their derivatives.
STEM_498_sm_SuppFig3.tif319KSupporting Information Figure 3. Relative competence for Brachyury induction by BMP. Relative expression levels of Brachyury in the presence or absence of BMP stimulation for 4 hours for the indicated cell types.
STEM_498_sm_SuppFig4.tif19382KSupporting Information Figure 4. Chimera formation potential of ES cell–derived EpiSCs. Blastocysts formed from ES cells or ES cell–derived EpiSCs aggregated with morulas.
STEM_498_sm_SuppFig5.tif2444KSupporting Information Figure 5. Microarray comparison of EpiSCs derived from embryonic epiblast or EpiInt cells. Scatter plot depicting the expression profile of epiblast stem cells derived from embryonic epiblast (EPId) versus that of differentiated ES cell–derived (ESd). Number of similar expressed genes: 44,873 (99.5℅); Correlation coefficient R; 0.985; Number of different expressed genes: 228 (0.506℅) (using a 2 fold change threshold in log2 scale).

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