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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 26 OCT 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 10, pages 1760–1771, October 2010
How to Cite
Belay, E., Mátrai, J., Acosta-Sanchez, A., Ma, L., Quattrocelli, M., Mátés, L., Sancho-Bru, P., Geraerts, M., Yan, B., Vermeesch, J., Rincón, M. Y., Samara-Kuko, E., Ivics, Z., Verfaillie, C., Sampaolesi, M., Izsvák, Z., VandenDriessche, T. and Chuah, M. K. L. (2010), Novel Hyperactive Transposons for Genetic Modification of Induced Pluripotent and Adult Stem Cells: A Nonviral Paradigm for Coaxed Differentiation. STEM CELLS, 28: 1760–1771. doi: 10.1002/stem.501
Author contributions: E.B.: designed the experiments, characterized the hyperactive transposases by transfection into iPS and adult stem cells, conducted the G-banding analysis, analyzed the data and wrote part of the manuscript; J.M.: performed immunohistochemistry staining, microscopy, FIX expression analysis, analyzed the data and wrote part of the manuscript; A.A.-S.: performed the molecular biology experiments and analyzed the data; L. Ma: designed the experiments and characterized the hyperactive transposases in myoblasts and MSC; M.Q.: designed and performed the Pax3-coaxed myogenic differentiation of iPS; L. Mátés: designed and generated the hyperactive transposases and transposons; P.S.-B.: designed and performed the hepatic and cardiomyogenic differentiation of iPS; M.G.: designed and performed the neural and glial differentiation of iPS; B.Y.: performed the adipogenic and myogenic differentiation experiments from MSC and myoblasts, respectively; J.V.: conducted the cytogenetic analysis and the array comparative genomic hybridization (aCGH) analysis; M.Y.R.: contributed to the cardiac differentiation experiments of iPS; E.S.-K.: performed molecular biology and cell culture experiments; Z.I.: designed and supervised the generation of the hyperactive transposases and transposons, interpreted the data; C.V.: designed and supervised the hepatic, cardiomyogenic, neural and glial differentiation experiments, interpreted the data; M.S.: designed and supervised the Pax3-coaxed myogenic differentiation experiments, interpreted the data; Z.I.: designed and supervised the generation of the hyperactive transposases and transposons, interpreted the data; T.V.: designed and supervised the experiments, interpreted the data and wrote the manuscript; M.K.L.C.: designed, supervised, and managed the experiments, interpreted the data, wrote the manuscript and coordinated the overall research effort. E.B. and J.M. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS August 16, 2010.
- Issue published online: 26 OCT 2010
- Article first published online: 26 OCT 2010
- Accepted manuscript online: 16 AUG 2010 12:00AM EST
- Manuscript Accepted: 5 AUG 2010
- Manuscript Received: 15 MAR 2010
Additional Supporting Information may be found in the online version of this article.
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