Adenomatous Polyposis Coli Is Essential for Both Neuronal Differentiation and Maintenance of Adult Neural Stem Cells in Subventricular Zone and Hippocampus

Additional Supporting Information may be found in the online version of this article.

Filename	Format	Size	Description
STEM_524_sm_suppinfoFig.eps		13187K	Figure 1. Non-CNS phenotypes of APC-CKO mice. A. Region-specific Cre-dependent deletion of the APC gene. The PCR analysis shows that only the undeleted alleles (314 bp) were amplified from the genomic DNA of APC580S/580S brain, CKO tail, and CKO liver, whereas both the undeleted and deleted alleles (258 bp) were amplified from that of CKO brain. B. Kaplan-Meier cumulative survival rate of CKO mice (n=37) and their control littermates (n=58). The mortality rate of CKO mice was significantly higher than that of littermate controls (Ctrl) (p<0.001, Mantel-Cox log-rank test). C. Postnatal growth retardation in CKO mice. A representative picture shows a littermate control mouse, a CKO mouse with mild growth retardation (CKO1), and that with severe growth retardation (CKO2). Body weights of CKO mice were significantly smaller than those of controls (*p<0.01 and **p<0.001 versus controls, t test). D. CKO mice with juvenile lethality exhibit hemorrhagic enteritis. Note hemorrhage in the jejunoileal segment (arrowhead). HE stained section shows erosion and inflammatory cell infiltration. E. Impaired lens development in CKO mice. A representative picture demonstrates a small eye phenotype in CKO mice. HE stained sections show that lens is small and disorganized whereas retinal layers are well preserved. Scale bars: D, E, 100 ?m. HE, hematoxylin
STEM_524_sm_suppinfo.doc		34K	Supporting Information

Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.