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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
STEM_537_sm_suppinfofigure1.tif5321KSupplementary figure 1. FACS controls. A/ Analysis of freshly sorted CD56+GFP− and CD56+GFP+ cells from a mixed population (50%/50%). B/ GFP analysis of cells sorted (CD56+GFP− or CD56+GFP+) and cloned (one cell by well in 96 well plates) using a FACS Aria cell sorter linked to an Automated Deposition Cell Unit. After 2 weeks of amplification, 30 clones were found to be fully GFP−, 40 clones were found to be GFP+ and no clones were found with a mixture of GFP+ and GFP− cells. Part of the total analysis is displayed under histogram or dot plot representation. C/ Example of compensation and isotype control assay for CD15−FITC and CD56−PE FACS analysis of cultivated muscle−derived cells.
STEM_537_sm_suppinfofigure2.tif741KSupplementary figure 2. A/ RT−PCR was performed to quantify the expression of various markers of adipogenesis or myogenesis from CD15+/−CD56+/− cells cultivated for 10 or 7 days in adipogenic or myogenic medium, respectively. UCP1 and CIDEA were amplified to investigate the presence of brown adipocytes. 18S rRNA and β-actin were amplified as controls. B/ RT−PCR analysis of CD34+CD15+CD56+, CD34+CD15−CD56+ and CD34−CD15−CD56+ sorted cells, freshly cultivated (passage 1) in proliferation medium, and prepared from b7 and b8 muscle samples. Whole muscle-derived cells were used as positive control. PAX7 and MYF5 were amplified as markers of the satellite cell lineage, and PRDM16 and MYF5 as markers of the muscle/brown adipocyte lineage. PW1 is a marker in mouse of both satellite cells and a subset of interstitial cells. 18S rRNA was amplified as control.
STEM_537_sm_suppinfofigure3.tif5474KSupplementary figure 3. Gallery of CD15−FITC (FL1−H) and CD56−PE (FL2−H) FACS profiles for freshly cultivated muscle-derived cells purified from healthy or pathological biopsies. Biopsies b1 to b8 correspond to biopsies described in Supplementary table 2. DM1 = type 1 myotonic dystrophy; FSH = facioscapulohumeral muscular dystrophy.
STEM_537_sm_suppinfotable1.tif538KSupporting Information Table 1
STEM_537_sm_suppinfotable2.tif256KSupporting Information Table 2

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