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Tissue-Specific Stem Cells
Version of Record online: 30 DEC 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 12, pages 2182–2194, December 2010
How to Cite
Pisani, D. F., Clement, N., Loubat, A., Plaisant, M., Sacconi, S., Kurzenne, J.-Y., Desnuelle, C., Dani, C. and Dechesne, C. A. (2010), Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle. STEM CELLS, 28: 2182–2194. doi: 10.1002/stem.537
Author contributions: D.F.P. and C.A.D.: collection and/or assembly of data, data analysis and interpretation, manuscript writing, conception and design, final approval of manuscript; C.D.: financial support, administrative support, data analysis and interpretation; N.C., A.L., and M.P.: collection and/or assembly of data; S.S., C.D., and J.Y.K.: collection and/or assembly of data, provision of study material or patients.
First published online in STEM CELLS EXPRESS October 8, 2010.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue online: 30 DEC 2010
- Version of Record online: 30 DEC 2010
- Accepted manuscript online: 8 OCT 2010 02:15PM EST
- Manuscript Accepted: 25 SEP 2010
- Manuscript Received: 27 APR 2010
- Centre National de la Recherche Scientifique
Additional Supporting Information may be found in the online version of this article.
|STEM_537_sm_suppinfofigure1.tif||5321K||Supplementary figure 1. FACS controls. A/ Analysis of freshly sorted CD56+GFP− and CD56+GFP+ cells from a mixed population (50%/50%). B/ GFP analysis of cells sorted (CD56+GFP− or CD56+GFP+) and cloned (one cell by well in 96 well plates) using a FACS Aria cell sorter linked to an Automated Deposition Cell Unit. After 2 weeks of amplification, 30 clones were found to be fully GFP−, 40 clones were found to be GFP+ and no clones were found with a mixture of GFP+ and GFP− cells. Part of the total analysis is displayed under histogram or dot plot representation. C/ Example of compensation and isotype control assay for CD15−FITC and CD56−PE FACS analysis of cultivated muscle−derived cells.|
|STEM_537_sm_suppinfofigure2.tif||741K||Supplementary figure 2. A/ RT−PCR was performed to quantify the expression of various markers of adipogenesis or myogenesis from CD15+/−CD56+/− cells cultivated for 10 or 7 days in adipogenic or myogenic medium, respectively. UCP1 and CIDEA were amplified to investigate the presence of brown adipocytes. 18S rRNA and β-actin were amplified as controls. B/ RT−PCR analysis of CD34+CD15+CD56+, CD34+CD15−CD56+ and CD34−CD15−CD56+ sorted cells, freshly cultivated (passage 1) in proliferation medium, and prepared from b7 and b8 muscle samples. Whole muscle-derived cells were used as positive control. PAX7 and MYF5 were amplified as markers of the satellite cell lineage, and PRDM16 and MYF5 as markers of the muscle/brown adipocyte lineage. PW1 is a marker in mouse of both satellite cells and a subset of interstitial cells. 18S rRNA was amplified as control.|
|STEM_537_sm_suppinfofigure3.tif||5474K||Supplementary figure 3. Gallery of CD15−FITC (FL1−H) and CD56−PE (FL2−H) FACS profiles for freshly cultivated muscle-derived cells purified from healthy or pathological biopsies. Biopsies b1 to b8 correspond to biopsies described in Supplementary table 2. DM1 = type 1 myotonic dystrophy; FSH = facioscapulohumeral muscular dystrophy.|
|STEM_537_sm_suppinfotable1.tif||538K||Supporting Information Table 1|
|STEM_537_sm_suppinfotable2.tif||256K||Supporting Information Table 2|
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