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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 30 DEC 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 12, pages 2141–2150, December 2010
How to Cite
Jeong, C.-H., Cho, Y.-Y., Kim, M.-O., Kim, S.-H., Cho, E.-J., Lee, S.-Y., Jeon, Y.-J., Yeong Lee, K., Yao, K., Keum, Y.-S., Bode, A. M. and Dong, Z. (2010), Phosphorylation of Sox2 Cooperates in Reprogramming to Pluripotent Stem Cells. STEM CELLS, 28: 2141–2150. doi: 10.1002/stem.540
Author contributions: C.-H.J.: conception and design, collection and/or assembly data, data analysis and interpretation, manuscript writing; Y.-Y.C.: conception and design; M.-O.K.: collection and/or assembly data; S.-H.K.: collection and/or assembly data; E.-J.C.: collection and/or assembly data; S.-Y.L.: collection and/or assembly data; Y.-J.J.: data analysis and interpretation; K.Y.L.: data analysis and interpretation; K.Y.: data analysis and interpretation; Y.-S.K.: data analysis and interpretation; A.M.B.: data analysis and interpretation, manuscript writing; Z.D.: conception and design, final approval of manuscript.
First published online in STEM CELLS EXPRESS October 13, 2010.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue published online: 30 DEC 2010
- Article first published online: 30 DEC 2010
- Accepted manuscript online: 13 OCT 2010 09:12AM EST
- Manuscript Accepted: 28 SEP 2010
- Manuscript Received: 13 APR 2010
- The Hormel Foundation, Mayo Foundation
- University of Minnesota
Vol. 30, Issue 9, 2064, Article first published online: 20 AUG 2012
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by transduction of reprogramming factors, including Oct4, Sox2, Klf4, and c-Myc. A coordinated network of these factors was suggested to confer a pluripotency of iPSCs. Together with Oct4, Sox2 plays a major role as a master regulator in ESCs. However, the underlying mechanisms by which Sox2 contributes to self-renewal or reprogramming processes remain to be determined. Here, we provide new evidence for a phosphorylation-based regulation of Sox2 activity. Akt directly interacts with Sox2 and promotes its stabilization through phosphorylation at Thr118, which enhances the transcriptional activity of Sox2 in ESCs. Moreover, phosphorylation of Sox2 cooperates in the reprogramming of mouse embryonic fibroblasts by enabling more efficient induction of iPSCs. Overall, our studies provide new insights into the regulatory mechanism of Sox2 in ESCs and also provide a direct link between phosphorylation events and somatic cell reprogramming. STEM CELLS 2010;28:2141–2150