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Translational and Clinical Research
Article first published online: 30 DEC 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 12, pages 2217–2228, December 2010
How to Cite
Kim, S. M., Oh, J. H., Park, S. A., Ryu, C. H., Lim, J. Y., Kim, D.-S., Chang, J. W., Oh, W. and Jeun, S.-S. (2010), Irradiation Enhances the Tumor Tropism and Therapeutic Potential of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Secreting Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Glioma Therapy. STEM CELLS, 28: 2217–2228. doi: 10.1002/stem.543
Author contrubutions: S.M.K.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, financial support; J.H.O.: collection and/or assembly of data, data analysis and interpretation; S.A.P.: collection and/or assembly of data; C.H.R.: data analysis and interpretation; J.Y.L.: data analysis and interpretation; D.-S.K. collection and/or assembly of data; J.W.C.: provision of study material, data analysis and interpretation; W.O.: provision of study material; S.-S.J.: conception and design, data analysis and interpretation, final approval of manuscript, financial support.
First published online in STEM CELLS EXPRESS October 13, 2010.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue published online: 30 DEC 2010
- Article first published online: 30 DEC 2010
- Accepted manuscript online: 13 OCT 2010 09:12AM EST
- Manuscript Accepted: 30 SEP 2010
- Manuscript Received: 28 MAY 2010
- National R&D Program for Cancer Control. Grant Number: 0820040
- Korea Healthcare Technology R&D Project. Grant Number: A091309
- Ministry for Health, Welfare and Family Affairs, Republic of Korea, and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology. Grant Number: 2010-0021527
|STEM_543_sm_suppinfofigure1.tif||8121K||Figure S1. Cytotoxicity of treatment with irradiation or rhTRAIL in human glioma cells. (A): Glioma cell lines (left panel) and primary glioma cells (right panel) were seeded in 96−well plates. Increasing doses of rhTRAIL (0–300 ng/ml) were treated to the wells. Cells were assayed for viability after 48 h by MTT assay. (B): Glioma cells were grown in 35 mm dishes overnight and then treated with increasing doses of irradiation (0–10 Gy) and analyzed after 48 h. Abbreviation: GBM, glioblastoma multiforme; rhTRAIL, recombinant human tumor necrosis factor−related apoptosis−inducing ligand.|
|STEM_543_sm_suppinfofigure2.tif||2724K||Figure S2. Synergistic effects of combined treatment with irradiation and rhTRAIL in glioma cells. Glioma cells were grown overnight and then treated with increasing doses of irradiation (0–10 Gy). For the effect of combined treatment, tumor cells or irradiated tumor cells were seeded in 24−well plates. Cells were cultured for 24 h after exposure to irradiation, followed by treatment with or without rhTRAIL of 10 ng/ml for U−87MG and GBM2 cells (A and B) or 100 ng/ml for U−373MG, U−251MG, GBM4, and GBM5 cells (C–F) for another 24 h. Cells were assayed for the viability using MTT assay. Abbreviation: GBM, glioblastoma multiforme; rhTRAIL, recombinant human tumor necrosis factor−related apoptosis−inducing ligand.|
|STEM_543_sm_suppinfofigure3.tif||8121K||Figure S3. Effect of fractionated radiation of glioma cells on the migration of MSC−TRAIL. (A): The migratory ability of MSC−TRAIL in response to CM from untreated and irradiated U−87MG glioma cells with single−fraction (2.5 and 10 Gy) or multiple fractions (2.5 Gy × 4) was determined using a Transwell plate (8 μm pores). Representative photomicrographs of stained filters show migrated cells. Magnification, ×200. (B): Cell migration was compared and evaluated after staining by taking photographs and counting cells that had migrated under a light microscope. *, P < .001 compared with untreated control.|
|STEM_543_sm_suppinfofigure4.tif||2722K||Figure S4. Expression levels of cytokines and upregulation of IL−8 mRNA in glioma cells after irradiation. (A): Glioma cells were irradiated with various doses and the expression levels of IL−8, SDF−1α, and MCP−1 in the culture supernatants were quantified by ELISA after 48 h. (B): IL−8 mRNA levels were determined in the cells from untreated or treated with irradiation. Abbreviation: IL−8, interleukin−8; SDF−1α, stromal cell−derived factor−1α; MCP−1, monocyte chemoattractant protein−1; GBM, glioblastoma multiforme; NHA, normal human astrocytes; GAPDH, glyceraldehyde−3−phosphate dehydrogenase.|
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