SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
STEM_543_sm_suppinfofigure1.tif8121KFigure S1. Cytotoxicity of treatment with irradiation or rhTRAIL in human glioma cells. (A): Glioma cell lines (left panel) and primary glioma cells (right panel) were seeded in 96−well plates. Increasing doses of rhTRAIL (0–300 ng/ml) were treated to the wells. Cells were assayed for viability after 48 h by MTT assay. (B): Glioma cells were grown in 35 mm dishes overnight and then treated with increasing doses of irradiation (0–10 Gy) and analyzed after 48 h. Abbreviation: GBM, glioblastoma multiforme; rhTRAIL, recombinant human tumor necrosis factor−related apoptosis−inducing ligand.
STEM_543_sm_suppinfofigure2.tif2724KFigure S2. Synergistic effects of combined treatment with irradiation and rhTRAIL in glioma cells. Glioma cells were grown overnight and then treated with increasing doses of irradiation (0–10 Gy). For the effect of combined treatment, tumor cells or irradiated tumor cells were seeded in 24−well plates. Cells were cultured for 24 h after exposure to irradiation, followed by treatment with or without rhTRAIL of 10 ng/ml for U−87MG and GBM2 cells (A and B) or 100 ng/ml for U−373MG, U−251MG, GBM4, and GBM5 cells (C–F) for another 24 h. Cells were assayed for the viability using MTT assay. Abbreviation: GBM, glioblastoma multiforme; rhTRAIL, recombinant human tumor necrosis factor−related apoptosis−inducing ligand.
STEM_543_sm_suppinfofigure3.tif8121KFigure S3. Effect of fractionated radiation of glioma cells on the migration of MSC−TRAIL. (A): The migratory ability of MSC−TRAIL in response to CM from untreated and irradiated U−87MG glioma cells with single−fraction (2.5 and 10 Gy) or multiple fractions (2.5 Gy × 4) was determined using a Transwell plate (8 μm pores). Representative photomicrographs of stained filters show migrated cells. Magnification, ×200. (B): Cell migration was compared and evaluated after staining by taking photographs and counting cells that had migrated under a light microscope. *, P < .001 compared with untreated control.
STEM_543_sm_suppinfofigure4.tif2722KFigure S4. Expression levels of cytokines and upregulation of IL−8 mRNA in glioma cells after irradiation. (A): Glioma cells were irradiated with various doses and the expression levels of IL−8, SDF−1α, and MCP−1 in the culture supernatants were quantified by ELISA after 48 h. (B): IL−8 mRNA levels were determined in the cells from untreated or treated with irradiation. Abbreviation: IL−8, interleukin−8; SDF−1α, stromal cell−derived factor−1α; MCP−1, monocyte chemoattractant protein−1; GBM, glioblastoma multiforme; NHA, normal human astrocytes; GAPDH, glyceraldehyde−3−phosphate dehydrogenase.
STEM_543_sm_suppinfo.doc26KSupporting Information

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.