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Translational and Clinical Research
Version of Record online: 30 DEC 2010
Copyright © 2010 AlphaMed Press
Volume 28, Issue 12, pages 2229–2238, December 2010
How to Cite
Krasnodembskaya, A., Song, Y., Fang, X., Gupta, N., Serikov, V., Lee, J.-W. and Matthay, M. A. (2010), Antibacterial Effect of Human Mesenchymal Stem Cells Is Mediated in Part from Secretion of the Antimicrobial Peptide LL-37. STEM CELLS, 28: 2229–2238. doi: 10.1002/stem.544
Author contributions: A.K.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; Y.S.: conception and design, collection and/or assembly of data, data analysis and interpretation; X.F.: conception and design, collection and/or assembly of data, data analysis and interpretation; N.G.: conception and design, financial support; V.S.: conception and design, collection and/or assembly of data; J.L.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing; M.A.M.: conception and design, financial and administrative support, data analysis and interpretation, manuscript writing, and final approval of manuscript.
First published online in STEM CELLS EXPRESS October 13, 2010.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue online: 30 DEC 2010
- Version of Record online: 30 DEC 2010
- Accepted manuscript online: 13 OCT 2010 09:12AM EST
- Manuscript Accepted: 30 SEP 2010
- Manuscript Received: 6 JUN 2010
- NHLBI. Grant Numbers: 51856, 51,854, 093026, HL092059
- NIAID. Grant Number: A1053194
- Texas A&M Health Science Center College of Medicine Institute for Regenerative Medicine at Scott & White through a grant from NCRR of the NIH, Grant. Grant Number: P40RR017447
Additional Supporting Information may be found in the online version of this article.
|STEM_544_sm_suppinfofigureS1.tif||3095K||Figure S1. MSC or its conditioned medium has antimicrobial activity against gram-positive bacteria. (A) MSC directly inhibits S.aureus growth after 6 hr co-incubation. MSC or NHLF were incubated with live S.aureus Newman strain for 6 hr. Bacterial growth was assessed by CFU counts. *P<0.003 vs. RPMI, √ p<0.0004 vs. NHLF by ANOVA (Bonferroni), n = 4. (B) The conditioned medium (CM) of MSC or NHLF with or without prior stimulation with live S.aureus was examined for antimicrobial activity. MSC CM after stimulation with S.aureus inhibited bacterial growth by 46% whereas CM from NHLF did not have a significant effect. Data are mean CFU ± SD, * P<0.002 vs. RPMI, √ p<0.0001 vs. NHLF CM (S.aureus stimulated) by ANOVA (Bonferroni), n = 4. Abbreviations: MSC, mesenchymal stem cells; NHLF, normal human lung fibroblasts; CFU, colony forming units; RPMI, RPMI-1640 medium.|
|STEM_544_sm_suppinfofigureS2.tif||3049K||Figure S2. Intra-tracheal administration of anti-LL 37 antibody alone had no effect in mouse E.coli pneumonia. (A) Administration of an anti-LL-37 neutralizing antibody (10 μg), as well as mouse IgG isotype antibody, did not change bacterial clearance in the BAL fluid compared to PBS treated mice. Values are mean CFU ± SD, n = 9. (B) Administration of neither anti-LL-37 antibody, nor mouse IgG isotype antibody, did not alter the antimicrobial activity of mouse BAL, compared to PBS treated mice. BAL samples were incubated with E.coli (105 CFU/ml) for 2 hr, and CFU growth was counted. Data are mean CFU ± SD, n = 8-9. Abbreviations: MSC, mesenchymal stem cells; BAL, bronchoalveolar lavage.|
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